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|a 10.1093/jxb/eraa402
|2 doi
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|a pubmed24n1224.xml
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|a DE-627
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|c DE-627
|e rakwb
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|a eng
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|a Lakhssassi, Naoufal
|e verfasserin
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|a Soybean TILLING-by-Sequencing+ reveals the role of novel GmSACPD members in unsaturated fatty acid biosynthesis while maintaining healthy nodules
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|c 2020
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 14.05.2021
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|a Date Revised 13.12.2023
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|a published: Print
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|a CommentIn: J Exp Bot. 2020 Dec 31;71(22):6857-6860. - PMID 33382894
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|a Citation Status MEDLINE
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|a © The Author(s) 2020. Published by Oxford University Press on behalf of the Society for Experimental Biology. All rights reserved. For permissions, please email: journals.permissionsoup.com.
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|a Developing soybean lines with high levels of stearic acid is a primary goal of the soybean industry. Most high-stearic-acid soybeans carry different GmSACPD-C mutated alleles. However, due to the dual role of GmSACPD-C in seeds and nodule development, all derived deleterious GmSACPD-C mutant alleles are of extremely poor agronomic value because of defective nodulation. The soybean stearoyl-acyl carrier protein desaturase (GmSACPD) gene family is composed of five members. Comparative genomics analysis indicated that SACPD genes were duplicated and derived from a common ancestor that is still present in chlorophytic algae. Synteny analysis showed the presence of segment duplications between GmSACPD-A/GmSACPD-B, and GmSACPD-C/GmSACPD-D. GmSACPD-E was not contained in any duplicated segment and may be the result of tandem duplication. We developed a TILLING by Target Capture Sequencing (Tilling-by-Sequencing+) technology, a versatile extension of the conventional TILLING by sequencing, and successfully identified 12, 14, and 18 ethyl methanesulfonate mutants at the GmSACPD-A, GmSACPD-B, and GmSACPD-D genes, respectively. Functional analysis of all identified mutants revealed an unprecedented role of GmSACPD-A, GmSACPD-B, and GmSACPD-D in unsaturated fatty acid biosynthesis without affecting nodule development and structure. This discovery will positively impact the development of high-stearic-acid lines to enhance soybean nutritional value without potential developmental tradeoffs
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a GmSACPD gene family
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|a Benchmarking
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|a TILLING-by-Sequencing+
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|a nodulation
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|a stearic acid
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|a subcellular localization
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|a subfunctionalization
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|a Fatty Acids, Unsaturated
|2 NLM
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|a Plant Proteins
|2 NLM
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|a Zhou, Zhou
|e verfasserin
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|a Liu, Shiming
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|a Piya, Sarbottam
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|a Cullen, Mallory A
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|a El Baze, Abdelhalim
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|a Knizia, Dounya
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|a Patil, Gunvant B
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|a Badad, Oussama
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|a Embaby, Mohamed G
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|a Meksem, Jonas
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|a Lakhssassi, Aicha
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|a AbuGhazaleh, Amer
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|a Hewezi, Tarek
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|a Meksem, Khalid
|e verfasserin
|4 aut
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|i Enthalten in
|t Journal of experimental botany
|d 1985
|g 71(2020), 22 vom: 31. Dez., Seite 6969-6987
|w (DE-627)NLM098182706
|x 1460-2431
|7 nnns
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|g volume:71
|g year:2020
|g number:22
|g day:31
|g month:12
|g pages:6969-6987
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|u http://dx.doi.org/10.1093/jxb/eraa402
|3 Volltext
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