Soybean TILLING-by-Sequencing+ reveals the role of novel GmSACPD members in unsaturated fatty acid biosynthesis while maintaining healthy nodules

© The Author(s) 2020. Published by Oxford University Press on behalf of the Society for Experimental Biology. All rights reserved. For permissions, please email: journals.permissionsoup.com.

Bibliographische Detailangaben
Veröffentlicht in:Journal of experimental botany. - 1985. - 71(2020), 22 vom: 31. Dez., Seite 6969-6987
1. Verfasser: Lakhssassi, Naoufal (VerfasserIn)
Weitere Verfasser: Zhou, Zhou, Liu, Shiming, Piya, Sarbottam, Cullen, Mallory A, El Baze, Abdelhalim, Knizia, Dounya, Patil, Gunvant B, Badad, Oussama, Embaby, Mohamed G, Meksem, Jonas, Lakhssassi, Aicha, AbuGhazaleh, Amer, Hewezi, Tarek, Meksem, Khalid
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2020
Zugriff auf das übergeordnete Werk:Journal of experimental botany
Schlagworte:Journal Article Research Support, Non-U.S. Gov't GmSACPD gene family Benchmarking TILLING-by-Sequencing+ nodulation stearic acid subcellular localization subfunctionalization Fatty Acids, Unsaturated Plant Proteins
LEADER 01000caa a22002652 4500
001 NLM314708790
003 DE-627
005 20231227130331.0
007 cr uuu---uuuuu
008 231225s2020 xx |||||o 00| ||eng c
024 7 |a 10.1093/jxb/eraa402  |2 doi 
028 5 2 |a pubmed24n1224.xml 
035 |a (DE-627)NLM314708790 
035 |a (NLM)32898219 
040 |a DE-627  |b ger  |c DE-627  |e rakwb 
041 |a eng 
100 1 |a Lakhssassi, Naoufal  |e verfasserin  |4 aut 
245 1 0 |a Soybean TILLING-by-Sequencing+ reveals the role of novel GmSACPD members in unsaturated fatty acid biosynthesis while maintaining healthy nodules 
264 1 |c 2020 
336 |a Text  |b txt  |2 rdacontent 
337 |a ƒaComputermedien  |b c  |2 rdamedia 
338 |a ƒa Online-Ressource  |b cr  |2 rdacarrier 
500 |a Date Completed 14.05.2021 
500 |a Date Revised 13.12.2023 
500 |a published: Print 
500 |a CommentIn: J Exp Bot. 2020 Dec 31;71(22):6857-6860. - PMID 33382894 
500 |a Citation Status MEDLINE 
520 |a © The Author(s) 2020. Published by Oxford University Press on behalf of the Society for Experimental Biology. All rights reserved. For permissions, please email: journals.permissionsoup.com. 
520 |a Developing soybean lines with high levels of stearic acid is a primary goal of the soybean industry. Most high-stearic-acid soybeans carry different GmSACPD-C mutated alleles. However, due to the dual role of GmSACPD-C in seeds and nodule development, all derived deleterious GmSACPD-C mutant alleles are of extremely poor agronomic value because of defective nodulation. The soybean stearoyl-acyl carrier protein desaturase (GmSACPD) gene family is composed of five members. Comparative genomics analysis indicated that SACPD genes were duplicated and derived from a common ancestor that is still present in chlorophytic algae. Synteny analysis showed the presence of segment duplications between GmSACPD-A/GmSACPD-B, and GmSACPD-C/GmSACPD-D. GmSACPD-E was not contained in any duplicated segment and may be the result of tandem duplication. We developed a TILLING by Target Capture Sequencing (Tilling-by-Sequencing+) technology, a versatile extension of the conventional TILLING by sequencing, and successfully identified 12, 14, and 18 ethyl methanesulfonate mutants at the GmSACPD-A, GmSACPD-B, and GmSACPD-D genes, respectively. Functional analysis of all identified mutants revealed an unprecedented role of GmSACPD-A, GmSACPD-B, and GmSACPD-D in unsaturated fatty acid biosynthesis without affecting nodule development and structure. This discovery will positively impact the development of high-stearic-acid lines to enhance soybean nutritional value without potential developmental tradeoffs 
650 4 |a Journal Article 
650 4 |a Research Support, Non-U.S. Gov't 
650 4 |a GmSACPD gene family 
650 4 |a Benchmarking 
650 4 |a TILLING-by-Sequencing+ 
650 4 |a nodulation 
650 4 |a stearic acid 
650 4 |a subcellular localization 
650 4 |a subfunctionalization 
650 7 |a Fatty Acids, Unsaturated  |2 NLM 
650 7 |a Plant Proteins  |2 NLM 
700 1 |a Zhou, Zhou  |e verfasserin  |4 aut 
700 1 |a Liu, Shiming  |e verfasserin  |4 aut 
700 1 |a Piya, Sarbottam  |e verfasserin  |4 aut 
700 1 |a Cullen, Mallory A  |e verfasserin  |4 aut 
700 1 |a El Baze, Abdelhalim  |e verfasserin  |4 aut 
700 1 |a Knizia, Dounya  |e verfasserin  |4 aut 
700 1 |a Patil, Gunvant B  |e verfasserin  |4 aut 
700 1 |a Badad, Oussama  |e verfasserin  |4 aut 
700 1 |a Embaby, Mohamed G  |e verfasserin  |4 aut 
700 1 |a Meksem, Jonas  |e verfasserin  |4 aut 
700 1 |a Lakhssassi, Aicha  |e verfasserin  |4 aut 
700 1 |a AbuGhazaleh, Amer  |e verfasserin  |4 aut 
700 1 |a Hewezi, Tarek  |e verfasserin  |4 aut 
700 1 |a Meksem, Khalid  |e verfasserin  |4 aut 
773 0 8 |i Enthalten in  |t Journal of experimental botany  |d 1985  |g 71(2020), 22 vom: 31. Dez., Seite 6969-6987  |w (DE-627)NLM098182706  |x 1460-2431  |7 nnns 
773 1 8 |g volume:71  |g year:2020  |g number:22  |g day:31  |g month:12  |g pages:6969-6987 
856 4 0 |u http://dx.doi.org/10.1093/jxb/eraa402  |3 Volltext 
912 |a GBV_USEFLAG_A 
912 |a SYSFLAG_A 
912 |a GBV_NLM 
912 |a GBV_ILN_350 
951 |a AR 
952 |d 71  |j 2020  |e 22  |b 31  |c 12  |h 6969-6987