Soybean TILLING-by-Sequencing+ reveals the role of novel GmSACPD members in unsaturated fatty acid biosynthesis while maintaining healthy nodules

Bibliographische Detailangaben
Veröffentlicht in:	Journal of experimental botany. - 1985. - 71(2020), 22 vom: 31. Dez., Seite 6969-6987
1. Verfasser:	Lakhssassi, Naoufal (VerfasserIn)
Weitere Verfasser:	Zhou, Zhou, Liu, Shiming, Piya, Sarbottam, Cullen, Mallory A, El Baze, Abdelhalim, Knizia, Dounya, Patil, Gunvant B, Badad, Oussama, Embaby, Mohamed G, Meksem, Jonas, Lakhssassi, Aicha, AbuGhazaleh, Amer, Hewezi, Tarek, Meksem, Khalid
Format:	Online-Aufsatz
Sprache:	English
Veröffentlicht:	2020
Zugriff auf das übergeordnete Werk:	Journal of experimental botany
Schlagworte:	Journal Article Research Support, Non-U.S. Gov't GmSACPD gene family Benchmarking TILLING-by-Sequencing+ nodulation stearic acid subcellular localization subfunctionalization Fatty Acids, Unsaturated Plant Proteins

Beschreibung
Zusammenfassung:	© The Author(s) 2020. Published by Oxford University Press on behalf of the Society for Experimental Biology. All rights reserved. For permissions, please email: journals.permissionsoup.com. Developing soybean lines with high levels of stearic acid is a primary goal of the soybean industry. Most high-stearic-acid soybeans carry different GmSACPD-C mutated alleles. However, due to the dual role of GmSACPD-C in seeds and nodule development, all derived deleterious GmSACPD-C mutant alleles are of extremely poor agronomic value because of defective nodulation. The soybean stearoyl-acyl carrier protein desaturase (GmSACPD) gene family is composed of five members. Comparative genomics analysis indicated that SACPD genes were duplicated and derived from a common ancestor that is still present in chlorophytic algae. Synteny analysis showed the presence of segment duplications between GmSACPD-A/GmSACPD-B, and GmSACPD-C/GmSACPD-D. GmSACPD-E was not contained in any duplicated segment and may be the result of tandem duplication. We developed a TILLING by Target Capture Sequencing (Tilling-by-Sequencing+) technology, a versatile extension of the conventional TILLING by sequencing, and successfully identified 12, 14, and 18 ethyl methanesulfonate mutants at the GmSACPD-A, GmSACPD-B, and GmSACPD-D genes, respectively. Functional analysis of all identified mutants revealed an unprecedented role of GmSACPD-A, GmSACPD-B, and GmSACPD-D in unsaturated fatty acid biosynthesis without affecting nodule development and structure. This discovery will positively impact the development of high-stearic-acid lines to enhance soybean nutritional value without potential developmental tradeoffs
Beschreibung:	Date Completed 14.05.2021 Date Revised 13.12.2023 published: Print CommentIn: J Exp Bot. 2020 Dec 31;71(22):6857-6860. - PMID 33382894 Citation Status MEDLINE
ISSN:	1460-2431
DOI:	10.1093/jxb/eraa402