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|a 10.1093/jxb/eraa391
|2 doi
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|a (NLM)32842152
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|a DE-627
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|e rakwb
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|a eng
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| 100 |
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|a Fayos, Ian
|e verfasserin
|4 aut
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|a Assessment of the roles of SPO11-2 and SPO11-4 in meiosis in rice using CRISPR/Cas9 mutagenesis
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|c 2020
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
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|2 rdamedia
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|a ƒa Online-Ressource
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|2 rdacarrier
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|a Date Completed 14.05.2021
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|a Date Revised 14.05.2021
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|a published: Print
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|a Citation Status MEDLINE
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|a © The Author(s) 2020. Published by Oxford University Press on behalf of the Society for Experimental Biology. All rights reserved. For permissions, please email: journals.permissionsoup.com.
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|a In Arabidopsis, chromosomal double-strand breaks at meiosis are presumably catalyzed by two distinct SPO11 transesterases, AtSPO11-1 and AtSPO11-2, together with M-TOPVIB. To clarify the roles of the SPO11 paralogs in rice, we used CRISPR/Cas9 mutagenesis to produce null biallelic mutants in OsSPO11-1, OsSPO11-2, and OsSPO11-4. Similar to Osspo11-1, biallelic mutations in the first exon of OsSPO11-2 led to complete panicle sterility. Conversely, all Osspo11-4 biallelic mutants were fertile. To generate segregating Osspo11-2 mutant lines, we developed a strategy based on dual intron targeting. Similar to Osspo11-1, the pollen mother cells of Osspo11-2 progeny plants showed an absence of bivalent formation at metaphase I, aberrant segregation of homologous chromosomes, and formation of non-viable tetrads. In contrast, the chromosome behavior in Osspo11-4 male meiocytes was indistinguishable from that in the wild type. While similar numbers of OsDMC1 foci were revealed by immunostaining in wild-type and Osspo11-4 prophase pollen mother cells (114 and 101, respectively), a surprisingly high number (85) of foci was observed in the sterile Osspo11-2 mutant, indicative of a divergent function between OsSPO11-1 and OsSPO11-2. This study demonstrates that whereas OsSPO11-1 and OsSPO11-2 are the likely orthologs of AtSPO11-1 and AtSPO11-2, OsSPO11-4 has no major role in wild-type rice meiosis
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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4 |
|a SPO11
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|a CRISPR/Cas9 mutagenesis
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|a meiosis
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| 650 |
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|a recombination
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| 650 |
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|a rice
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| 700 |
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|a Meunier, Anne Cécile
|e verfasserin
|4 aut
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| 700 |
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|a Vernet, Aurore
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Navarro-Sanz, Sergi
|e verfasserin
|4 aut
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| 700 |
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|a Portefaix, Murielle
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Lartaud, Marc
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Bastianelli, Giacomo
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Périn, Christophe
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Nicolas, Alain
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Guiderdoni, Emmanuel
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Journal of experimental botany
|d 1985
|g 71(2020), 22 vom: 31. Dez., Seite 7046-7058
|w (DE-627)NLM098182706
|x 1460-2431
|7 nnns
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| 773 |
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|g volume:71
|g year:2020
|g number:22
|g day:31
|g month:12
|g pages:7046-7058
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|u http://dx.doi.org/10.1093/jxb/eraa391
|3 Volltext
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