RPA-PCR couple : an approach to expedite plant diagnostics and overcome PCR inhibitors
DNA extraction can be lengthy and sometimes ends up with amplification inhibitors. We present the potential of recombinase polymerase amplification (RPA) to replace plant DNA extraction. In our rapid 'RPA-PCR couple' concept, RPA is tuned to slower reaction kinetics to promote amplificatio...
Ausführliche Beschreibung
Bibliographische Detailangaben
| Veröffentlicht in: | BioTechniques. - 1988. - 69(2020), 4 vom: 18. Okt., Seite 270-280
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| 1. Verfasser: |
Munawar, Mustafa Ahmad
(VerfasserIn) |
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| Weitere Verfasser: |
Martin, Frank,
Toljamo, Anna,
Kokko, Harri,
Oksanen, Elina |
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| Format: | Online-Aufsatz
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| Sprache: | English |
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| Veröffentlicht: |
2020
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| Zugriff auf das übergeordnete Werk: | BioTechniques
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| Schlagworte: | Journal Article
Research Support, Non-U.S. Gov't
PCR inhibitors
Phytophthora
Phytophthora cactorum
Phytophthora fragariae
atp9-nad9
plant DNA extraction
recombinase polymerase amplification
Benzothiazoles
mehr...
DNA, Plant
Diamines
Quinolines
Recombinases
SYBR Green I
163795-75-3 |
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