RPA-PCR couple : an approach to expedite plant diagnostics and overcome PCR inhibitors

DNA extraction can be lengthy and sometimes ends up with amplification inhibitors. We present the potential of recombinase polymerase amplification (RPA) to replace plant DNA extraction. In our rapid 'RPA-PCR couple' concept, RPA is tuned to slower reaction kinetics to promote amplificatio...

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Publié dans:BioTechniques. - 1991. - 69(2020), 4 vom: 02. Okt., Seite 270-280
Auteur principal: Munawar, Mustafa Ahmad (Auteur)
Autres auteurs: Martin, Frank, Toljamo, Anna, Kokko, Harri, Oksanen, Elina
Format: Article en ligne
Langue:English
Publié: 2020
Accès à la collection:BioTechniques
Sujets:Journal Article Research Support, Non-U.S. Gov't PCR inhibitors Phytophthora Phytophthora cactorum Phytophthora fragariae atp9-nad9 plant DNA extraction recombinase polymerase amplification Benzothiazoles plus... DNA, Plant Diamines Quinolines Recombinases SYBR Green I 163795-75-3