A Cyclometalated Iridium (III) Complex as a Microtubule Probe for Correlative Super-Resolution Fluorescence and Electron Microscopy

Détails bibliographiques
Publié dans:	Advanced materials (Deerfield Beach, Fla.). - 1998. - 32(2020), 39 vom: 10. Okt., Seite e2003901
Auteur principal:	Tian, Xiaohe (Auteur)
Autres auteurs:	De Pace, Cesare, Ruiz-Perez, Lorena, Chen, Bo, Su, Rina, Zhang, Mingzhu, Zhang, Ruilong, Zhang, Qiong, Wang, Qin, Zhou, Hongping, Wu, Jieying, Zhang, Zhongping, Tian, Yupeng, Battaglia, Giuseppe
Format:	Article en ligne
Langue:	English
Publié:	2020
Accès à la collection:	Advanced materials (Deerfield Beach, Fla.)
Sujets:	Journal Article correlation light-electron microscopy microtubules organometallic probes super-resolution microscopy Coordination Complexes Iridium 44448S9773

Description
Résumé:	© 2020 Wiley-VCH GmbH. The visualization of microtubules by combining optical and electron microscopy techniques provides valuable information to understand correlated intracellular activities. However, the lack of appropriate probes to bridge both microscopic resolutions restricts the areas and structures that can be comprehended within such highly assembled structures. Here, a versatile cyclometalated iridium (III) complex is designed that achieves synchronous fluorescence-electron microscopy correlation. The selective insertion of the probe into a microtubule triggers remarkable fluorescence enhancement and promising electron contrast. The long-life, highly photostable probe allows live-cell super-resolution imaging of tubulin localization and motion with a resolution of ≈30 nm. Furthermore, correlative light-electron microscopy and energy-filtered transmission electron microscopy reveal the well-associated optical and electron signal at a high specificity, with an interspace of ≈41 Å of microtubule monomer in cells
Description:	Date Completed 11.08.2021 Date Revised 11.08.2021 published: Print-Electronic Citation Status MEDLINE
ISSN:	1521-4095
DOI:	10.1002/adma.202003901