A Cyclometalated Iridium (III) Complex as a Microtubule Probe for Correlative Super-Resolution Fluorescence and Electron Microscopy

A Cyclometalated Iridium (III) Complex as a Microtubule Probe for Correlative Super-Resolution Fluorescence and Electron Microscopy

© 2020 Wiley-VCH GmbH.

Bibliographische Detailangaben
Veröffentlicht in:Advanced materials (Deerfield Beach, Fla.). - 1998. - 32(2020), 39 vom: 10. Okt., Seite e2003901
1. Verfasser: Tian, Xiaohe (VerfasserIn)
Weitere Verfasser: De Pace, Cesare, Ruiz-Perez, Lorena, Chen, Bo, Su, Rina, Zhang, Mingzhu, Zhang, Ruilong, Zhang, Qiong, Wang, Qin, Zhou, Hongping, Wu, Jieying, Zhang, Zhongping, Tian, Yupeng, Battaglia, Giuseppe
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2020
Zugriff auf das übergeordnete Werk:Advanced materials (Deerfield Beach, Fla.)
Schlagworte:Journal Article correlation light-electron microscopy microtubules organometallic probes super-resolution microscopy Coordination Complexes Iridium 44448S9773
Beschreibung
Zusammenfassung:© 2020 Wiley-VCH GmbH.
The visualization of microtubules by combining optical and electron microscopy techniques provides valuable information to understand correlated intracellular activities. However, the lack of appropriate probes to bridge both microscopic resolutions restricts the areas and structures that can be comprehended within such highly assembled structures. Here, a versatile cyclometalated iridium (III) complex is designed that achieves synchronous fluorescence-electron microscopy correlation. The selective insertion of the probe into a microtubule triggers remarkable fluorescence enhancement and promising electron contrast. The long-life, highly photostable probe allows live-cell super-resolution imaging of tubulin localization and motion with a resolution of ≈30 nm. Furthermore, correlative light-electron microscopy and energy-filtered transmission electron microscopy reveal the well-associated optical and electron signal at a high specificity, with an interspace of ≈41 Å of microtubule monomer in cells
Beschreibung:Date Completed 11.08.2021
Date Revised 11.08.2021
published: Print-Electronic
Citation Status MEDLINE
ISSN:1521-4095
DOI:10.1002/adma.202003901