Directional auxin fluxes in plants by intramolecular domain-domain coevolution of PIN auxin transporters
©2020 The Authors. New Phytologist ©2020 New Phytologist Trust.
| Veröffentlicht in: | The New phytologist. - 1979. - 227(2020), 5 vom: 15. Sept., Seite 1406-1416 |
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| 1. Verfasser: | |
| Weitere Verfasser: | , , |
| Format: | Online-Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
2020
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| Zugriff auf das übergeordnete Werk: | The New phytologist |
| Schlagworte: | Journal Article Research Support, Non-U.S. Gov't PIN auxin transporters cellular polarity directional auxin flow domain swapping domain-domain coevolution subcellular membrane localization Arabidopsis Proteins Indoleacetic Acids mehr... |
| Zusammenfassung: | ©2020 The Authors. New Phytologist ©2020 New Phytologist Trust. Morphogenesis and adaptive tropic growth in plants depend on gradients of the phytohormone auxin, mediated by the membrane-based PIN-FORMED (PIN) auxin transporters. PINs localize to a particular side of the plasma membrane (PM) or to the endoplasmic reticulum (ER) to directionally transport auxin and maintain intercellular and intracellular auxin homeostasis, respectively. However, the molecular cues that confer their diverse cellular localizations remain largely unknown. In this study, we systematically swapped the domains between ER- and PM-localized PIN proteins, as well as between apical and basal PM-localized PINs from Arabidopsis thaliana, to shed light on why PIN family members with similar topological structures reside at different membrane compartments within cells. Our results show that not only do the N- and C-terminal transmembrane domains (TMDs) and central hydrophilic loop contribute to their differential subcellular localizations and cellular polarity, but that the pairwise-matched N- and C-terminal TMDs resulting from intramolecular domain-domain coevolution are also crucial for their divergent patterns of localization. These findings illustrate the complexity of the evolutionary path of PIN proteins in acquiring their plethora of developmental functions and adaptive growth in plants |
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| Beschreibung: | Date Completed 14.05.2021 Date Revised 14.05.2021 published: Print-Electronic Citation Status MEDLINE |
| ISSN: | 1469-8137 |
| DOI: | 10.1111/nph.16629 |