Effect of photocatalysis (TiO2/UVA) on the inactivation and inhibition of Pseudomonas aeruginosa virulence factors expression
Water disinfection using visible light-active photocatalyst has recently attracted more attention due to its potential to inactivate microbes. In this study, we have investigated the efficiency of photocatalysis (TiO2/UVA) on the inactivation of Pseudomonas aeruginosa and the attenuation of its viru...
| Publié dans: | Environmental technology. - 1993. - 42(2021), 27 vom: 03. Nov., Seite 4237-4246 |
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| Auteur principal: | |
| Autres auteurs: | , , , , , |
| Format: | Article en ligne |
| Langue: | English |
| Publié: |
2021
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| Accès à la collection: | Environmental technology |
| Sujets: | Journal Article P. aeruginosa inactivation Photocatalysis biofilm virulence water treatment Anti-Bacterial Agents Virulence Factors titanium dioxide 15FIX9V2JP plus... |
| Résumé: | Water disinfection using visible light-active photocatalyst has recently attracted more attention due to its potential to inactivate microbes. In this study, we have investigated the efficiency of photocatalysis (TiO2/UVA) on the inactivation of Pseudomonas aeruginosa and the attenuation of its virulence factors. For this aim, the photocatalytic effects of TiO2/UVA on the cultivability and viability of P. aeruginosa were investigated. Furthermore, during the photocatalysis, the morphology of the bacterial cells was examined by atomic force microscopy (AFM) while the virulence factors were assessed by protease and lipase activities in addition to the mobility and communication of cells. The results revealed that during the photocatalysis the bacterial cells lost their cultivability and viability on agar under the action of the reactive oxygen species generated by the photocatalytic reaction. In addition, AFM observations have shown a damage of the bacterial membrane and a total disruption of the bacterial cells. Moreover, the major virulence factors such as biofilm, lipase and protease expression have been markedly inhibited by TiO2/UVA treatment. In addition, the bacteria lost their ability of communication 'quorum sensing' and mobility with twitching and swarming types after 60 min of photocatalytic treatment. Accordingly, TiO2/UVA is an effective method to reduce P. aeruginosa virulence and to prevent biofilm formation |
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| Description: | Date Completed 10.11.2021 Date Revised 10.11.2021 published: Print-Electronic Citation Status MEDLINE |
| ISSN: | 1479-487X |
| DOI: | 10.1080/09593330.2020.1751729 |