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231225s2020 xx |||||o 00| ||eng c |
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|a 10.1111/nph.16338
|2 doi
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|a pubmed24n1012.xml
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|a (DE-627)NLM303722126
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|a (NLM)31769874
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Liu, Zhenhua
|e verfasserin
|4 aut
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| 245 |
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|a Drivers of metabolic diversification
|b how dynamic genomic neighbourhoods generate new biosynthetic pathways in the Brassicaceae
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|c 2020
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 14.05.2021
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|a Date Revised 16.07.2022
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|a published: Print-Electronic
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|a CommentIn: New Phytol. 2020 Aug;227(4):992-994. - PMID 32433781
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|a Citation Status MEDLINE
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|a © 2019 The Authors. New Phytologist © 2019 New Phytologist Trust.
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|a Plants produce an array of specialized metabolites with important ecological functions. The mechanisms underpinning the evolution of new biosynthetic pathways are not well-understood. Here, we exploit available genome sequence resources to investigate triterpene biosynthesis across the Brassicaceae. Oxidosqualene cyclases (OSCs) catalyze the first committed step in triterpene biosynthesis. Systematic analysis of 13 sequenced Brassicaceae genomes was performed to identify all OSC genes. The genome neighbourhoods (GNs) around a total of 163 OSC genes were investigated to identify Pfam domains significantly enriched in these regions. All-vs-all comparisons of OSC neighbourhoods and phylogenomic analysis were used to investigate the sequence similarity and evolutionary relationships of the numerous candidate triterpene biosynthetic gene clusters (BGCs) observed. Functional analysis of three representative BGCs was carried out and their triterpene pathway products were elucidated. Our results indicate that plant genomes are remarkably plastic, and that dynamic GNs generate new biosynthetic pathways in different Brassicaceae lineages by shuffling the genes encoding a core palette of triterpene-diversifying enzymes, presumably in response to strong environmental selection pressure. These results illuminate a genomic basis for diversification of plant-specialized metabolism through natural combinatorics of enzyme families, which can be mimicked using synthetic biology to engineer diverse bioactive molecules
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|a Journal Article
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|a Research Support, N.I.H., Extramural
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|a Research Support, Non-U.S. Gov't
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| 650 |
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4 |
|a Brassicaceae
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| 650 |
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4 |
|a biosynthetic gene clusters
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| 650 |
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4 |
|a metabolic pathway evolution
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| 650 |
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4 |
|a plant interactions
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| 650 |
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4 |
|a specialized metabolism
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| 650 |
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4 |
|a terpenes
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| 700 |
1 |
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|a Suarez Duran, Hernando G
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Harnvanichvech, Yosapol
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Stephenson, Michael J
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Schranz, M Eric
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Nelson, David
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Medema, Marnix H
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Osbourn, Anne
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t The New phytologist
|d 1979
|g 227(2020), 4 vom: 01. Aug., Seite 1109-1123
|w (DE-627)NLM09818248X
|x 1469-8137
|7 nnns
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| 773 |
1 |
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|g volume:227
|g year:2020
|g number:4
|g day:01
|g month:08
|g pages:1109-1123
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|u http://dx.doi.org/10.1111/nph.16338
|3 Volltext
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|d 227
|j 2020
|e 4
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|c 08
|h 1109-1123
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