Drivers of metabolic diversification how dynamic genomic neighbourhoods generate new biosynthetic pathways in the Brassicaceae

Drivers of metabolic diversification : how dynamic genomic neighbourhoods generate new biosynthetic pathways in the Brassicaceae

© 2019 The Authors. New Phytologist © 2019 New Phytologist Trust.

Bibliographische Detailangaben
Veröffentlicht in:The New phytologist. - 1979. - 227(2020), 4 vom: 01. Aug., Seite 1109-1123
1. Verfasser: Liu, Zhenhua (VerfasserIn)
Weitere Verfasser: Suarez Duran, Hernando G, Harnvanichvech, Yosapol, Stephenson, Michael J, Schranz, M Eric, Nelson, David, Medema, Marnix H, Osbourn, Anne
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2020
Zugriff auf das übergeordnete Werk:The New phytologist
Schlagworte:Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Brassicaceae biosynthetic gene clusters metabolic pathway evolution plant interactions specialized metabolism terpenes
Beschreibung
Zusammenfassung:© 2019 The Authors. New Phytologist © 2019 New Phytologist Trust.
Plants produce an array of specialized metabolites with important ecological functions. The mechanisms underpinning the evolution of new biosynthetic pathways are not well-understood. Here, we exploit available genome sequence resources to investigate triterpene biosynthesis across the Brassicaceae. Oxidosqualene cyclases (OSCs) catalyze the first committed step in triterpene biosynthesis. Systematic analysis of 13 sequenced Brassicaceae genomes was performed to identify all OSC genes. The genome neighbourhoods (GNs) around a total of 163 OSC genes were investigated to identify Pfam domains significantly enriched in these regions. All-vs-all comparisons of OSC neighbourhoods and phylogenomic analysis were used to investigate the sequence similarity and evolutionary relationships of the numerous candidate triterpene biosynthetic gene clusters (BGCs) observed. Functional analysis of three representative BGCs was carried out and their triterpene pathway products were elucidated. Our results indicate that plant genomes are remarkably plastic, and that dynamic GNs generate new biosynthetic pathways in different Brassicaceae lineages by shuffling the genes encoding a core palette of triterpene-diversifying enzymes, presumably in response to strong environmental selection pressure. These results illuminate a genomic basis for diversification of plant-specialized metabolism through natural combinatorics of enzyme families, which can be mimicked using synthetic biology to engineer diverse bioactive molecules
Beschreibung:Date Completed 14.05.2021
Date Revised 16.07.2022
published: Print-Electronic
CommentIn: New Phytol. 2020 Aug;227(4):992-994. - PMID 32433781
Citation Status MEDLINE
ISSN:1469-8137
DOI:10.1111/nph.16338