Populus euphratica WRKY1 binds the promoter of H+-ATPase gene to enhance gene expression and salt tolerance

Populus euphratica WRKY1 binds the promoter of H+-ATPase gene to enhance gene expression and salt tolerance

© The Author(s) 2020. Published by Oxford University Press on behalf of the Society for Experimental Biology.

Bibliographische Detailangaben
Veröffentlicht in:Journal of experimental botany. - 1985. - 71(2020), 4 vom: 19. Feb., Seite 1527-1539
1. Verfasser: Yao, Jun (VerfasserIn)
Weitere Verfasser: Shen, Zedan, Zhang, Yanli, Wu, Xia, Wang, Jianhui, Sa, Gang, Zhang, Yuhong, Zhang, Huilong, Deng, Chen, Liu, Jian, Hou, Siyuan, Zhang, Ying, Zhang, Yinan, Zhao, Nan, Deng, Shurong, Lin, Shanzhi, Zhao, Rui, Chen, Shaoliang
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2020
Zugriff auf das übergeordnete Werk:Journal of experimental botany
Schlagworte:Journal Article Research Support, Non-U.S. Gov't DNA affinity purification sequencing H+-ATPase NaCl PeWRKY1 W-box electrophoretic mobility shift assay luciferase reporter assay non-invasive microtest technique mehr... virus-induced gene silencing DNA-Binding Proteins Plant Proteins Transcription Factors Proton-Translocating ATPases EC 3.6.3.14
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500 |a Citation Status MEDLINE 
520 |a © The Author(s) 2020. Published by Oxford University Press on behalf of the Society for Experimental Biology. 
520 |a Plasma membrane proton pumps play a crucial role in maintaining ionic homeostasis in salt-resistant Populus euphratica under saline conditions. High levels of NaCl (200 mM) induced PeHA1 expression in P. euphratica roots and leaves. We isolated a 2022 bp promoter fragment upstream of the translational start of PeHA1 from P. euphratica. The promoter-reporter construct PeHA1-pro::GUS was transferred to tobacco plants, demonstrating that β-glucuronidase activities increased in root, leaf, and stem tissues under salt stress. DNA affinity purification sequencing revealed that PeWRKY1 protein targeted the PeHA1 gene. We assessed the salt-induced transcriptional response of PeWRKY1 and its interaction with PeHA1 in P. euphratica. PeWRKY1 binding to the PeHA1 W-box in the promoter region was verified by a yeast one-hybrid assay, EMSA, luciferase reporter assay, and virus-induced gene silencing. Transgenic tobacco plants overexpressing PeWRKY1 had improved expression of NtHA4, which has a cis-acting W-box in the regulatory region, and improved H+ pumping activity in both in vivo and in vitro assays. We conclude that salt stress up-regulated PeHA1 transcription due to the binding of PeWRKY1 to the W-box in the promoter region of PeHA1. Thus, we conclude that enhanced H+ pumping activity enabled salt-stressed plants to retain Na+ homeostasis 
650 4 |a Journal Article 
650 4 |a Research Support, Non-U.S. Gov't 
650 4 |a DNA affinity purification sequencing 
650 4 |a H+-ATPase 
650 4 |a NaCl 
650 4 |a PeWRKY1 
650 4 |a W-box 
650 4 |a electrophoretic mobility shift assay 
650 4 |a luciferase reporter assay 
650 4 |a non-invasive microtest technique 
650 4 |a virus-induced gene silencing 
650 7 |a DNA-Binding Proteins  |2 NLM 
650 7 |a Plant Proteins  |2 NLM 
650 7 |a Transcription Factors  |2 NLM 
650 7 |a Proton-Translocating ATPases  |2 NLM 
650 7 |a EC 3.6.3.14  |2 NLM 
700 1 |a Shen, Zedan  |e verfasserin  |4 aut 
700 1 |a Zhang, Yanli  |e verfasserin  |4 aut 
700 1 |a Wu, Xia  |e verfasserin  |4 aut 
700 1 |a Wang, Jianhui  |e verfasserin  |4 aut 
700 1 |a Sa, Gang  |e verfasserin  |4 aut 
700 1 |a Zhang, Yuhong  |e verfasserin  |4 aut 
700 1 |a Zhang, Huilong  |e verfasserin  |4 aut 
700 1 |a Deng, Chen  |e verfasserin  |4 aut 
700 1 |a Liu, Jian  |e verfasserin  |4 aut 
700 1 |a Hou, Siyuan  |e verfasserin  |4 aut 
700 1 |a Zhang, Ying  |e verfasserin  |4 aut 
700 1 |a Zhang, Yinan  |e verfasserin  |4 aut 
700 1 |a Zhao, Nan  |e verfasserin  |4 aut 
700 1 |a Deng, Shurong  |e verfasserin  |4 aut 
700 1 |a Lin, Shanzhi  |e verfasserin  |4 aut 
700 1 |a Zhao, Rui  |e verfasserin  |4 aut 
700 1 |a Chen, Shaoliang  |e verfasserin  |4 aut 
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