Populus euphratica WRKY1 binds the promoter of H+-ATPase gene to enhance gene expression and salt tolerance

Bibliographische Detailangaben
Veröffentlicht in:	Journal of experimental botany. - 1985. - 71(2020), 4 vom: 19. Feb., Seite 1527-1539
1. Verfasser:	Yao, Jun (VerfasserIn)
Weitere Verfasser:	Shen, Zedan, Zhang, Yanli, Wu, Xia, Wang, Jianhui, Sa, Gang, Zhang, Yuhong, Zhang, Huilong, Deng, Chen, Liu, Jian, Hou, Siyuan, Zhang, Ying, Zhang, Yinan, Zhao, Nan, Deng, Shurong, Lin, Shanzhi, Zhao, Rui, Chen, Shaoliang
Format:	Online-Aufsatz
Sprache:	English
Veröffentlicht:	2020
Zugriff auf das übergeordnete Werk:	Journal of experimental botany
Schlagworte:	Journal Article Research Support, Non-U.S. Gov't DNA affinity purification sequencing H+-ATPase NaCl PeWRKY1 W-box electrophoretic mobility shift assay luciferase reporter assay non-invasive microtest technique mehr... virus-induced gene silencing DNA-Binding Proteins Plant Proteins Transcription Factors Proton-Translocating ATPases EC 3.6.3.14

Beschreibung
Zusammenfassung:	© The Author(s) 2020. Published by Oxford University Press on behalf of the Society for Experimental Biology. Plasma membrane proton pumps play a crucial role in maintaining ionic homeostasis in salt-resistant Populus euphratica under saline conditions. High levels of NaCl (200 mM) induced PeHA1 expression in P. euphratica roots and leaves. We isolated a 2022 bp promoter fragment upstream of the translational start of PeHA1 from P. euphratica. The promoter-reporter construct PeHA1-pro::GUS was transferred to tobacco plants, demonstrating that β-glucuronidase activities increased in root, leaf, and stem tissues under salt stress. DNA affinity purification sequencing revealed that PeWRKY1 protein targeted the PeHA1 gene. We assessed the salt-induced transcriptional response of PeWRKY1 and its interaction with PeHA1 in P. euphratica. PeWRKY1 binding to the PeHA1 W-box in the promoter region was verified by a yeast one-hybrid assay, EMSA, luciferase reporter assay, and virus-induced gene silencing. Transgenic tobacco plants overexpressing PeWRKY1 had improved expression of NtHA4, which has a cis-acting W-box in the regulatory region, and improved H+ pumping activity in both in vivo and in vitro assays. We conclude that salt stress up-regulated PeHA1 transcription due to the binding of PeWRKY1 to the W-box in the promoter region of PeHA1. Thus, we conclude that enhanced H+ pumping activity enabled salt-stressed plants to retain Na+ homeostasis
Beschreibung:	Date Completed 14.05.2021 Date Revised 31.05.2022 published: Print Dryad: 10.5061/dryad.83ng085 Citation Status MEDLINE
ISSN:	1460-2431
DOI:	10.1093/jxb/erz493