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231225s2019 xx |||||o 00| ||eng c |
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|a 10.1021/acs.langmuir.9b01822
|2 doi
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|a pubmed24n1000.xml
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|a (NLM)31394036
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Pyle, Joseph R
|e verfasserin
|4 aut
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| 245 |
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|a In Situ Sensing of Reactive Oxygen Species on Dye-Stained Single DNA Molecules under Illumination
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|c 2019
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
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|2 rdamedia
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|a ƒa Online-Ressource
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|2 rdacarrier
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|a Date Completed 11.08.2020
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|a Date Revised 03.09.2020
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Reactive oxygen species (ROS) are a necessary evil in many biological systems and have been measured with fluorescent probes at the ensemble levels both in vitro and in vivo. Measuring ROS generated from a single molecule is important for mechanistic studies, yet measuring ROS near a dye-labeled single-molecule under illumination has been challenging. Here, we use CellROX, a group of ROS probes, to sense ROS near dye-stained DNA that has been flow-stretched and immobilized on a surface. ROS is responsible for the photodamage of DNA molecules under this circumstance. In this report, we confirmed the ROS sensing reaction in bulk solutions and optimized the conditions for single-molecule experiments including the selection of substrates, dye concentrations, probes in the CellROX series, excitation lasers, and emission filter-sets. We observed a correlation between ROS and the dye-labeled DNA and localized the ROS-activated CellROX probe molecules at both the ensemble level and the single-molecule level
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|a Journal Article
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|a Research Support, N.I.H., Extramural
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|a Fluorescent Dyes
|2 NLM
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|a Reactive Oxygen Species
|2 NLM
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|a DNA
|2 NLM
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|a 9007-49-2
|2 NLM
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|a Sy Piecco, Kurt Waldo E
|e verfasserin
|4 aut
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|a Vicente, Juvinch R
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Chen, Jixin
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1992
|g 35(2019), 35 vom: 03. Sept., Seite 11308-11314
|w (DE-627)NLM098181009
|x 1520-5827
|7 nnns
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| 773 |
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|g volume:35
|g year:2019
|g number:35
|g day:03
|g month:09
|g pages:11308-11314
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|u http://dx.doi.org/10.1021/acs.langmuir.9b01822
|3 Volltext
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|h 11308-11314
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