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231225s2019 xx |||||o 00| ||eng c |
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|a 10.1111/jfd.13051
|2 doi
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|a pubmed24n0995.xml
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|a (NLM)31241770
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|a DE-627
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|e rakwb
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|a eng
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| 100 |
1 |
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|a Bethke, Jorn
|e verfasserin
|4 aut
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| 245 |
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|a Renibacterium salmoninarum iron-acquisition mechanisms and ASK cell line infection
|b Virulence and immune response
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|c 2019
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
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|2 rdacarrier
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|a Date Completed 28.01.2020
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|a Date Revised 09.12.2020
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|a published: Print-Electronic
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|a GENBANK: NC_010168.1
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|a Citation Status MEDLINE
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|a © 2019 John Wiley & Sons Ltd.
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|a Renibacterium salmoninarum is the aetiological agent of bacterial kidney disease (BKD) in salmonid farms. This pathogen possesses at least three iron-acquisition mechanisms, but the link between these mechanisms and virulence is unclear. Therefore, this study used RT-qPCR to assess the effects of normal and iron-limited conditions on iron-uptake genes controlled by IdeR and related to iron acquisition in Chilean R. salmoninarum strain H-2 and the type strain DSM20767T . Further evaluated was the in vitro immune-related response of the Atlantic Salmon Kidney (ASK) cell line, derived from the primary organ affected by BKD. R. salmoninarum grown under iron-limited conditions overexpressed genes involved in haemin uptake and siderophore transport, with overexpression significantly higher in H-2 than DSM20767T . These overexpressed genes resulted in higher cytotoxicity and an increased immune response (i.e., TNF-α, IL-1β, TLR1 and INF-γ) in the ASK cell line. This response was significantly higher against bacteria grown under iron-limited conditions, especially H-2. These observations indicate that iron-acquisition mechanisms are possibly highly related to the virulence and pathogenic capacity of R. salmoninarum. In conclusion, treatments that block iron-uptake mechanisms or siderophore synthesis are attractive therapeutic approaches for treating R. salmoninarum, which causes significant aquaculture losses
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|a Journal Article
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|a R. salmoninarum
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| 650 |
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|a ASK
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|a BKD
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| 650 |
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|a immune response
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| 650 |
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|a iron
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|a siderophore
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| 650 |
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|a Iron
|2 NLM
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| 650 |
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|a E1UOL152H7
|2 NLM
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| 700 |
1 |
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|a Arias-Muñoz, Eloisa
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Yáñez, Alejandro
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Avendaño-Herrera, Ruben
|e verfasserin
|4 aut
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| 773 |
0 |
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|i Enthalten in
|t Journal of fish diseases
|d 1998
|g 42(2019), 9 vom: 01. Sept., Seite 1283-1291
|w (DE-627)NLM098166034
|x 1365-2761
|7 nnns
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| 773 |
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|g volume:42
|g year:2019
|g number:9
|g day:01
|g month:09
|g pages:1283-1291
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|u http://dx.doi.org/10.1111/jfd.13051
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