Renibacterium salmoninarum iron-acquisition mechanisms and ASK cell line infection Virulence and immune response

Renibacterium salmoninarum iron-acquisition mechanisms and ASK cell line infection : Virulence and immune response

© 2019 John Wiley & Sons Ltd.

Bibliographische Detailangaben
Veröffentlicht in:Journal of fish diseases. - 1998. - 42(2019), 9 vom: 01. Sept., Seite 1283-1291
1. Verfasser: Bethke, Jorn (VerfasserIn)
Weitere Verfasser: Arias-Muñoz, Eloisa, Yáñez, Alejandro, Avendaño-Herrera, Ruben
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2019
Zugriff auf das übergeordnete Werk:Journal of fish diseases
Schlagworte:Journal Article R. salmoninarum ASK BKD immune response iron siderophore Iron E1UOL152H7
Beschreibung
Zusammenfassung:© 2019 John Wiley & Sons Ltd.
Renibacterium salmoninarum is the aetiological agent of bacterial kidney disease (BKD) in salmonid farms. This pathogen possesses at least three iron-acquisition mechanisms, but the link between these mechanisms and virulence is unclear. Therefore, this study used RT-qPCR to assess the effects of normal and iron-limited conditions on iron-uptake genes controlled by IdeR and related to iron acquisition in Chilean R. salmoninarum strain H-2 and the type strain DSM20767T . Further evaluated was the in vitro immune-related response of the Atlantic Salmon Kidney (ASK) cell line, derived from the primary organ affected by BKD. R. salmoninarum grown under iron-limited conditions overexpressed genes involved in haemin uptake and siderophore transport, with overexpression significantly higher in H-2 than DSM20767T . These overexpressed genes resulted in higher cytotoxicity and an increased immune response (i.e., TNF-α, IL-1β, TLR1 and INF-γ) in the ASK cell line. This response was significantly higher against bacteria grown under iron-limited conditions, especially H-2. These observations indicate that iron-acquisition mechanisms are possibly highly related to the virulence and pathogenic capacity of R. salmoninarum. In conclusion, treatments that block iron-uptake mechanisms or siderophore synthesis are attractive therapeutic approaches for treating R. salmoninarum, which causes significant aquaculture losses
Beschreibung:Date Completed 28.01.2020
Date Revised 09.12.2020
published: Print-Electronic
GENBANK: NC_010168.1
Citation Status MEDLINE
ISSN:1365-2761
DOI:10.1111/jfd.13051