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231225s2019 xx |||||o 00| ||eng c |
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|a 10.1107/S1600576719001900
|2 doi
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|a DE-627
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|e rakwb
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|a eng
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1 |
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|a Sharma, Amit
|e verfasserin
|4 aut
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| 245 |
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|a A simple adaptation to a protein crystallography station to facilitate difference X-ray scattering studies
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|c 2019
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
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|a Date Revised 15.07.2024
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|a published: Electronic-eCollection
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|a Citation Status PubMed-not-MEDLINE
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|a The X-ray crystallography station I911-2 at MAXLab II (Lund, Sweden) has been adapted to enable difference small- and wide-angle X-ray scattering (SAXS/WAXS) data to be recorded. Modifications to the beamline included a customized flow cell, a motorized flow cell holder, a helium cone, a beam stop, a sample stage and a sample delivery system. This setup incorporated external devices such as infrared lasers, LEDs and reaction mixers to induce conformational changes in macromolecules. This platform was evaluated through proof-of-principle experiments capturing light-induced conformational changes in phytochromes. A difference WAXS signature of conformational changes in a plant aqua-porin was also demonstrated using caged calcium
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|a Journal Article
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|a difference X-ray scattering
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|a protein structural changes
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|a Berntsen, Peter
|e verfasserin
|4 aut
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| 700 |
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|a Harimoorthy, Rajiv
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Appio, Roberto
|e verfasserin
|4 aut
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|a Sjöhamn, Jennie
|e verfasserin
|4 aut
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| 700 |
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|a Järvå, Michael
|e verfasserin
|4 aut
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| 700 |
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|a Björling, Alexander
|e verfasserin
|4 aut
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| 700 |
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|a Hammarin, Greger
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Westenhoff, Sebastian
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Brändén, Gisela
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Neutze, Richard
|e verfasserin
|4 aut
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| 773 |
0 |
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|i Enthalten in
|t Journal of applied crystallography
|d 1998
|g 52(2019), Pt 2 vom: 01. Apr., Seite 378-386
|w (DE-627)NLM098121561
|x 0021-8898
|7 nnns
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| 773 |
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|g volume:52
|g year:2019
|g number:Pt 2
|g day:01
|g month:04
|g pages:378-386
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|u http://dx.doi.org/10.1107/S1600576719001900
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|d 52
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