A simple adaptation to a protein crystallography station to facilitate difference X-ray scattering studies

The X-ray crystallography station I911-2 at MAXLab II (Lund, Sweden) has been adapted to enable difference small- and wide-angle X-ray scattering (SAXS/WAXS) data to be recorded. Modifications to the beamline included a customized flow cell, a motorized flow cell holder, a helium cone, a beam stop,...

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Détails bibliographiques
Publié dans:Journal of applied crystallography. - 1998. - 52(2019), Pt 2 vom: 01. Apr., Seite 378-386
Auteur principal: Sharma, Amit (Auteur)
Autres auteurs: Berntsen, Peter, Harimoorthy, Rajiv, Appio, Roberto, Sjöhamn, Jennie, Järvå, Michael, Björling, Alexander, Hammarin, Greger, Westenhoff, Sebastian, Brändén, Gisela, Neutze, Richard
Format: Article en ligne
Langue:English
Publié: 2019
Accès à la collection:Journal of applied crystallography
Sujets:Journal Article difference X-ray scattering protein structural changes
Description
Résumé:The X-ray crystallography station I911-2 at MAXLab II (Lund, Sweden) has been adapted to enable difference small- and wide-angle X-ray scattering (SAXS/WAXS) data to be recorded. Modifications to the beamline included a customized flow cell, a motorized flow cell holder, a helium cone, a beam stop, a sample stage and a sample delivery system. This setup incorporated external devices such as infrared lasers, LEDs and reaction mixers to induce conformational changes in macromolecules. This platform was evaluated through proof-of-principle experiments capturing light-induced conformational changes in phytochromes. A difference WAXS signature of conformational changes in a plant aqua-porin was also demonstrated using caged calcium
Description:Date Revised 15.07.2024
published: Electronic-eCollection
Citation Status PubMed-not-MEDLINE
ISSN:0021-8898
DOI:10.1107/S1600576719001900