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231225s2019 xx |||||o 00| ||eng c |
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|a 10.2144/btn-2018-0147
|2 doi
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|a pubmed24n0986.xml
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|a (DE-627)NLM296082740
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|a (NLM)30987444
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Zolotarev, Nikolay
|e verfasserin
|4 aut
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| 245 |
1 |
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|a Removal of extra sequences with I-SceI in combination with CRISPR/Cas9 technique for precise gene editing in Drosophila
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|c 2019
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| 336 |
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 31.01.2020
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|a Date Revised 31.01.2020
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|a published: Print
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|a Citation Status MEDLINE
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|a The CRISPR/Cas9 system has recently emerged as a powerful tool for functional genomic studies and has been adopted for many organisms, including Drosophila. Previously, an efficient two-step strategy was developed to engineer the fly genome by combining CRISPR/Cas9 with recombinase-mediated cassette exchange (RMCE). This strategy allows the introduction of designed mutations into a gene of interest in vivo. However, the loxP or frt site remains in the edited locus. Here, we propose a modification of this approach for rapid and efficient seamless genome editing with CRISPR/Cas9 and site-specific recombinase-mediated integration (SSRMI) combined with recombination between homologous sequences induced by the rare-cutting endonuclease I-SceI. The induced homological recombination leads to the removal of the remaining extraneous sequences from the target locus
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a CRISPR/Cas9
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4 |
|a genome editing
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| 650 |
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4 |
|a homologous recombination
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| 650 |
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|a Drosophila Proteins
|2 NLM
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| 650 |
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|a Recombinases
|2 NLM
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| 650 |
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|a Deoxyribonuclease I
|2 NLM
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| 650 |
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|a EC 3.1.21.1
|2 NLM
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| 700 |
1 |
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|a Georgiev, Pavel
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Maksimenko, Oksana
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t BioTechniques
|d 1988
|g 66(2019), 4 vom: 01. Apr., Seite 198-201
|w (DE-627)NLM012627046
|x 1940-9818
|7 nnns
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| 773 |
1 |
8 |
|g volume:66
|g year:2019
|g number:4
|g day:01
|g month:04
|g pages:198-201
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| 856 |
4 |
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|u http://dx.doi.org/10.2144/btn-2018-0147
|3 Volltext
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|d 66
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