Removal of extra sequences with I-SceI in combination with CRISPR/Cas9 technique for precise gene editing in Drosophila
The CRISPR/Cas9 system has recently emerged as a powerful tool for functional genomic studies and has been adopted for many organisms, including Drosophila. Previously, an efficient two-step strategy was developed to engineer the fly genome by combining CRISPR/Cas9 with recombinase-mediated cassette...
| Veröffentlicht in: | BioTechniques. - 1988. - 66(2019), 4 vom: 01. Apr., Seite 198-201 |
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| Format: | Online-Aufsatz |
| Sprache: | English |
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2019
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| Zugriff auf das übergeordnete Werk: | BioTechniques |
| Schlagworte: | Journal Article Research Support, Non-U.S. Gov't CRISPR/Cas9 genome editing homologous recombination Drosophila Proteins Recombinases Deoxyribonuclease I EC 3.1.21.1 |
| Online verfügbar |
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