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231225s2019 xx |||||o 00| ||eng c |
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|a 10.1093/jxb/erz081
|2 doi
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|a pubmed25n0982.xml
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|e rakwb
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|a eng
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|a Chen, Jiajing
|e verfasserin
|4 aut
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|a Cit1,2RhaT and two novel CitdGlcTs participate in flavor-related flavonoid metabolism during citrus fruit development
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|c 2019
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 06.07.2020
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|a Date Revised 06.07.2020
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|a published: Print
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|a Citation Status MEDLINE
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|a © The Author(s) 2019. Published by Oxford University Press on behalf of the Society for Experimental Biology.
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|a Neohesperidosides are disaccharides that are present in some flavonoids and impart a bitter taste, which can significantly affect the commercial value of citrus fruits. In this study, we identified three flavonoid-7-O-di-glucosyltransferase (dGlcT) genes closely related to 1,2-rhamnosyltransferase (1,2RhaT) in citrus genomes. However, only 1,2RhaT was directly linked to the accumulation of neohesperidoside, as demonstrated by association analysis of 50 accessions and co-segregation analysis of an F1 population derived from Citrus reticulata × Poncirus trifoliata. In transgenic tobacco BY2 cells, over-expression of CitdGlcTs resulted in flavonoid-7-O-glucosides being catalysed into bitterless flavonoid-7-O-di-glucosides, whereas over-expression of Cit1,2RhaT converted the same substrate into bitter-tasting flavonoid-7-O-neohesperidoside. Unlike 1,2RhaT, during citrus fruit development the dGlcTs showed an opposite expression pattern to CHS and CHI, two genes encoding rate-limiting enzymes of flavonoid biosynthesis. An uncoupled availability of dGlcTs and substrates might result in trace accumulation of flavonoid-7-O-di-glucosides in the fruit of C. maxima (pummelo). Past human selection of the deletion and functional mutation of 1,2RhaT has led step-by-step to the evolution of the flavor-related metabolic network in citrus. Our research provides the basis for potentially improving the taste in citrus fruit through manipulation of the network by knocking-out 1,2RhaT or by enhancing the expression of dGlcT using genetic transformation
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Bitterness
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|a citrus
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|a flavonoid
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|a flavonoid-7-O-di-glucosides
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|a flavonoid-7-O-glucoside
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|a neohesperidoside
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|a Flavonoids
|2 NLM
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|a Yuan, Ziyu
|e verfasserin
|4 aut
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|a Zhang, Haipeng
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|a Li, Wenyun
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|a Shi, Meiyan
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|a Peng, Zhaoxin
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|a Li, Mingyue
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|a Tian, Jing
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|a Deng, Xiuxin
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|a Cheng, Yunjiang
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|a Deng, Cecilia Hong
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|a Xie, Zongzhou
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|a Zeng, Jiwu
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|a Yao, Jia-Long
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|a Xu, Juan
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Journal of experimental botany
|d 1985
|g 70(2019), 10 vom: 09. Mai, Seite 2759-2771
|w (DE-627)NLM098182706
|x 1460-2431
|7 nnns
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| 773 |
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|g volume:70
|g year:2019
|g number:10
|g day:09
|g month:05
|g pages:2759-2771
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|u http://dx.doi.org/10.1093/jxb/erz081
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