A convenient method to generate and maintain poly(A)-encoding DNA sequences required for in vitro transcription of mRNA

Generating mRNA in vitro to encode therapeutic or cell-modifying proteins is rapidly gaining favor. An important factor that determines efficiency of translation from in vitro transcribed mRNA is the length of the 3' poly(A) sequence. However, reproducibly generating and maintaining templates f...

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Veröffentlicht in:	BioTechniques. - 1991. - 66(2019), 1 vom: 15. Jan., Seite 37-39
1. Verfasser:	Arbuthnot, Patrick (VerfasserIn)
Weitere Verfasser:	Ely, Abdullah, Bloom, Kristie
Format:	Online-Aufsatz
Sprache:	English
Veröffentlicht:	2019
Zugriff auf das übergeordnete Werk:	BioTechniques
Schlagworte:	Journal Article Research Support, Non-U.S. Gov't transcription mRNA plasmid poly(A) type IIS restriction enzymes DNA, Circular RNA, Messenger Poly A mehr... 24937-83-5 endodeoxyribonuclease SapI EC 3.1.21.- endodeoxyribonuclease ScaI Deoxyribonucleases, Type II Site-Specific EC 3.1.21.4