A convenient method to generate and maintain poly(A)-encoding DNA sequences required for in vitro transcription of mRNA

Generating mRNA in vitro to encode therapeutic or cell-modifying proteins is rapidly gaining favor. An important factor that determines efficiency of translation from in vitro transcribed mRNA is the length of the 3' poly(A) sequence. However, reproducibly generating and maintaining templates f...

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Publié dans:	BioTechniques. - 1991. - 66(2019), 1 vom: 15. Jan., Seite 37-39
Auteur principal:	Arbuthnot, Patrick (Auteur)
Autres auteurs:	Ely, Abdullah, Bloom, Kristie
Format:	Article en ligne
Langue:	English
Publié:	2019
Accès à la collection:	BioTechniques
Sujets:	Journal Article Research Support, Non-U.S. Gov't transcription mRNA plasmid poly(A) type IIS restriction enzymes DNA, Circular RNA, Messenger Poly A plus... 24937-83-5 endodeoxyribonuclease SapI EC 3.1.21.- endodeoxyribonuclease ScaI Deoxyribonucleases, Type II Site-Specific EC 3.1.21.4