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231225s2019 xx |||||o 00| ||eng c |
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|a 10.1021/acs.langmuir.8b03682
|2 doi
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|a pubmed24n0973.xml
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|a (NLM)30576146
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Zhou, Lele
|e verfasserin
|4 aut
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| 245 |
1 |
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|a Construction of a Liquid Crystal-Based Sensing Platform for Sensitive and Selective Detection of l-Phenylalanine Based on Alkaline Phosphatase
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|c 2019
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
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|2 rdamedia
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|a ƒa Online-Ressource
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|2 rdacarrier
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|a Date Revised 20.11.2019
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|a published: Print-Electronic
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|a Citation Status PubMed-not-MEDLINE
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|a The detection of l-phenylalanine (l-Phe) has become one of the most pressing issues concerning diagnosis and treatment of phenylketonuria in neonates; however, a simple and robust methodology is yet to be developed. Here, the application of novel liquid crystals (LCs)-sensing platform for sensitive, selective, and label-free detection of l-Phe was reported at the first time. We devised a strategy to fabricate the sodium monododecyl phosphate (SMP)-decorated LC sensing platform with the appearance of dark. Then, a dark to bright (D-B) optical images alteration of LCs was observed after transferring alkaline phosphatase (ALP) to the interface, owing to cleavage of SMP induced by ALP. LCs remained dark images after the SMP-decorated interface in contact with the pre-incubated ALP and l-Phe. Such optical appearance resulted from the inhibition of ALP by l-Phe, which was further verified by the isothermal titration calorimetry (ITC). The strategy was applied to sensing l-Phe, which have been proven to allow for sensitively and selectively differentiation of l-Phe from interfering compounds with similar aromatic groups, as well as seven other essential amino acids. More importantly, the detection limit of l-Phe reached 1 pg/mL in urine samples, further demonstrating its value in the practical applications. Results obtained in this study clearly demonstrated the superiority of LCs toward the l-Phe detection, which can pave a way for the development of high performance and robust probes for l-Phe detection in clinical applications
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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| 700 |
1 |
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|a Hu, Qiongzheng
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Kang, Qi
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Fang, Ming
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Yu, Li
|e verfasserin
|4 aut
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| 773 |
0 |
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|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1992
|g 35(2019), 2 vom: 15. Jan., Seite 461-467
|w (DE-627)NLM098181009
|x 1520-5827
|7 nnns
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| 773 |
1 |
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|g volume:35
|g year:2019
|g number:2
|g day:15
|g month:01
|g pages:461-467
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| 856 |
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|u http://dx.doi.org/10.1021/acs.langmuir.8b03682
|3 Volltext
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