Flow cytometric assessment of leukocyte kinetics for the monitoring of tissue damage

Flow cytometric assessment of leukocyte kinetics for the monitoring of tissue damage

Copyright © 2018. Published by Elsevier Inc.

Bibliographische Detailangaben
Veröffentlicht in:Clinical immunology (Orlando, Fla.). - 1999. - 197(2018) vom: 15. Dez., Seite 224-230
1. Verfasser: van den Bossche, Wouter B L (VerfasserIn)
Weitere Verfasser: Rykov, Kyrill, Teodosio, Cristina, Ten Have, Bas L E F, Knobben, Bas A S, Sietsma, Maurits S, Josiassen, Karin, de Bruin-Versteeg, Sandra, Orfao, Alberto, van Dongen, Jacques J M, van Raay, Jos J A M
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2018
Zugriff auf das übergeordnete Werk:Clinical immunology (Orlando, Fla.)
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Flow cytometry and multiparameter analysis Immune monitoring Leukocytes Tissue damage
Beschreibung
Zusammenfassung:Copyright © 2018. Published by Elsevier Inc.
Leukocyte populations quickly respond to tissue damage, but most leukocyte kinetic studies are not based on multiparameter flow cytometry. We systematically investigated several blood leukocyte populations after controlled tissue damage. 48 patients were assigned to either an anterior or posterolateral total hip arthroplasty. Peripheral blood was collected pre-operatively and at 2 h, 24 h, 48 h, 2 and 6 weeks postoperatively and assessed by flow cytometry for absolute counts of multiple leukocyte populations using standardized EuroFlow protocols. Absolute counts of leukocyte subsets differed significantly between consecutive time points. Neutrophils increased instantly after surgery, while most leukocyte subsets initially decreased, followed by increasing cell counts until 48 h. At two weeks all leukocyte counts were restored to pre-operative counts. Immune cell kinetics upon acute tissue damage exhibit reproducible patterns, which differ between the leukocyte subsets and with "opposite kinetics" among monocyte subsets. Flow cytometric leukocyte monitoring can be used to minimally invasively monitor tissue damage
Beschreibung:Date Completed 07.10.2019
Date Revised 07.10.2019
published: Print-Electronic
ErratumIn: Clin Immunol. 2020 Apr;213:108344. - PMID 31964597
Citation Status MEDLINE
ISSN:1521-7035
DOI:10.1016/j.clim.2018.09.014