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|a eng
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|a Santana-Calvo, Carmen
|e verfasserin
|4 aut
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|a Robust evaluation of intermolecular FRET using a large Stokes shift fluorophore as a donor
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|c 2018
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
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|a Date Completed 05.08.2019
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|a Date Revised 05.08.2019
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|a published: Print
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|a Citation Status MEDLINE
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|a Fluorescence (or Förster) resonance energy transfer (FRET) is a straightforward and sensitive technique to evaluate molecular interactions. However, most of the popular FRET pairs suffer cross-excitation of the acceptor, which could lead to false positives. To overcome this problem, we selected a large Stokes shift (LSS) fluorophore as a FRET donor. As a successful example, we employed a new FRET pair mAmetrine (an LSS yellow fluorescence protein)/DY-547 (a cyanine derivative) to substitute CFP/fluorescein that we previously employed to study molecular interactions between cyclic nucleotide-binding domains and cyclic nucleotides. The new FRET pair is practically free of cross-excitation of the acceptor. Namely, a change in the fluorescence spectral shape implies evidence of FRET without other control experiments
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|a binding assay
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|a fluorescent protein
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|a intermolecular FRET
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|a large Stokes shift
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|a Guanine Nucleotide Exchange Factors
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|a Romero, Francisco
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|a López-González, Ignacio
|e verfasserin
|4 aut
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|a Nishigaki, Takuya
|e verfasserin
|4 aut
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|i Enthalten in
|t BioTechniques
|d 1993
|g 65(2018), 4 vom: 02. Okt., Seite 211-218
|w (DE-627)NLM012627046
|x 1940-9818
|7 nnns
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| 773 |
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|g volume:65
|g year:2018
|g number:4
|g day:02
|g month:10
|g pages:211-218
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