Robust evaluation of intermolecular FRET using a large Stokes shift fluorophore as a donor
Fluorescence (or Förster) resonance energy transfer (FRET) is a straightforward and sensitive technique to evaluate molecular interactions. However, most of the popular FRET pairs suffer cross-excitation of the acceptor, which could lead to false positives. To overcome this problem, we selected a la...
Ausführliche Beschreibung
Bibliographische Detailangaben
| Veröffentlicht in: | BioTechniques. - 1993. - 65(2018), 4 vom: 02. Okt., Seite 211-218
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| 1. Verfasser: |
Santana-Calvo, Carmen
(VerfasserIn) |
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| Weitere Verfasser: |
Romero, Francisco,
López-González, Ignacio,
Nishigaki, Takuya |
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| Format: | Online-Aufsatz
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| Sprache: | English |
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| Veröffentlicht: |
2018
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| Zugriff auf das übergeordnete Werk: | BioTechniques
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| Schlagworte: | Journal Article
Research Support, Non-U.S. Gov't
binding assay
fluorescent protein
intermolecular FRET
large Stokes shift
Fluorescent Dyes
Guanine Nucleotide Exchange Factors
Luminescent Proteins
RAPGEF3 protein, human
mehr...
Cyclic AMP
E0399OZS9N |
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