Directional high-throughput sequencing of RNAs without gene-specific primers
Ribosomal RNA analysis is a useful tool for characterization of microbial communities. However, the lack of broad-range primers has hampered the simultaneous analysis of eukaryotic and prokaryotic members by amplicon sequencing. We present a complete workflow for directional, primer-independent sequ...
| Veröffentlicht in: | BioTechniques. - 1993. - 65(2018), 4 vom: 02. Okt., Seite 219-223 |
|---|---|
| 1. Verfasser: | |
| Weitere Verfasser: | |
| Format: | Online-Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
2018
|
| Zugriff auf das übergeordnete Werk: | BioTechniques |
| Schlagworte: | Journal Article Research Support, Non-U.S. Gov't algae ligation next-generation sequencing phytoplankton primer bias random priming ribosomal RNA DNA Primers mehr... |
| Online verfügbar |
Volltext |