Directional high-throughput sequencing of RNAs without gene-specific primers
Ribosomal RNA analysis is a useful tool for characterization of microbial communities. However, the lack of broad-range primers has hampered the simultaneous analysis of eukaryotic and prokaryotic members by amplicon sequencing. We present a complete workflow for directional, primer-independent sequ...
| Publié dans: | BioTechniques. - 1991. - 65(2018), 4 vom: 02. Okt., Seite 219-223 |
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| Format: | Article en ligne |
| Langue: | English |
| Publié: |
2018
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| Accès à la collection: | BioTechniques |
| Sujets: | Journal Article Research Support, Non-U.S. Gov't algae ligation next-generation sequencing phytoplankton primer bias random priming ribosomal RNA DNA Primers plus... |
| Accès en ligne |
Volltext |