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|a 10.2144/btn-2018-0074
|2 doi
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|e rakwb
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|a eng
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| 100 |
1 |
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|a Texier, Pauline
|e verfasserin
|4 aut
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| 245 |
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|a A useful gene cassette for conditional knock-down of essential genes by targeted promoter replacement in Mycobacteria
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|c 2018
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| 336 |
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
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|2 rdamedia
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|a ƒa Online-Ressource
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|2 rdacarrier
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|a Date Completed 31.07.2019
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|a Date Revised 31.07.2019
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|a published: Print
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|a Citation Status MEDLINE
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|a A direct method to study essential genes is to construct conditional knock-down mutants by replacement of their native promoter by an inducible one. In Mycobacteria, replacement of an essential gene promoter with an anhydrotetracycline inducible one was successfully used but required a multi-step approach. In this work, we describe a gene cassette for the engineering of a conditional knock-down mutant, which allows the one-step targeted replacement of mycobacterial promoters by an anhydrotetracycline-inducible promoter. The functionality of this cassette was successfully tested by engineering conditional clpP and SecA1 mutants of Mycobacterium smegmatis
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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4 |
|a ClpP
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| 650 |
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4 |
|a Mycobacterium smegmatis
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| 650 |
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4 |
|a SecA1
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| 650 |
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4 |
|a promoter replacement
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| 650 |
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4 |
|a recombineering
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| 650 |
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4 |
|a tetracycline promoter
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| 650 |
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7 |
|a Tetracyclines
|2 NLM
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| 650 |
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|a 4-epianhydrotetracycline
|2 NLM
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| 650 |
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7 |
|a 680VDL31MX
|2 NLM
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| 700 |
1 |
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|a Coddeville, Michèle
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Bordes, Patricia
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Genevaux, Pierre
|e verfasserin
|4 aut
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| 773 |
0 |
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|i Enthalten in
|t BioTechniques
|d 1993
|g 65(2018), 3 vom: 18. Sept., Seite 159-162
|w (DE-627)NLM012627046
|x 1940-9818
|7 nnns
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| 773 |
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|g volume:65
|g year:2018
|g number:3
|g day:18
|g month:09
|g pages:159-162
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|u http://dx.doi.org/10.2144/btn-2018-0074
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