A useful gene cassette for conditional knock-down of essential genes by targeted promoter replacement in Mycobacteria
A direct method to study essential genes is to construct conditional knock-down mutants by replacement of their native promoter by an inducible one. In Mycobacteria, replacement of an essential gene promoter with an anhydrotetracycline inducible one was successfully used but required a multi-step ap...
| Veröffentlicht in: | BioTechniques. - 1993. - 65(2018), 3 vom: 18. Sept., Seite 159-162 |
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| Format: | Online-Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
2018
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| Zugriff auf das übergeordnete Werk: | BioTechniques |
| Schlagworte: | Journal Article Research Support, Non-U.S. Gov't ClpP Mycobacterium smegmatis SecA1 promoter replacement recombineering tetracycline promoter Tetracyclines 4-epianhydrotetracycline |
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