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231225s2019 xx |||||o 00| ||eng c |
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|a 10.1021/acs.langmuir.8b00632
|2 doi
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|a pubmed24n0952.xml
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|a (NLM)29940726
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Ohara, Yuki
|e verfasserin
|4 aut
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| 245 |
1 |
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|a pH-Responsive Polyion Complex Vesicle with Polyphosphobetaine Shells
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|c 2019
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| 336 |
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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| 338 |
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|a ƒa Online-Ressource
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|2 rdacarrier
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|a Date Revised 20.11.2019
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|a published: Print-Electronic
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|a Citation Status PubMed-not-MEDLINE
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|a When a bioactive molecule is taken into cells by endocytosis, it is sometimes unable to escape from the lysosomes, resulting in inefficient drug release. We prepared pH-responsive polyion complex (PIC) vesicles that collapse under acidic conditions such as those inside a lysosome. Furthermore, under acidic conditions, cationic polymer was released from the PIC vesicles to break the lysosome membranes. Diblock copolymers (P20M167 and P20A190) consisting of water-soluble zwitterionic poly(2-methacryloyloxyethyl phosphorylcholine) (PMPC) block and cationic or anionic blocks were synthesized via reversible addition-fragmentation chain transfer (RAFT) radical polymerization. Poly(3-(methacrylamidopropyl) trimethylammonium chloride) (PMAPTAC) and poly(sodium 6-acrylamidohexanoate) (PAaH) were used as the cationic and anionic blocks, respectively. The pendant hexanoate groups in the PAaH block are ionized in basic water and in phosphate buffered saline (PBS), while the hexanoate groups are protonated in acidic water. In basic water, PIC vesicles were formed from a charge neutralized mixture of oppositely charged diblock copolymers. At the interface of PIC vesicle and water exists biocompatible PMPC shells. Under acidic conditions, the PIC vesicles collapsed, because the charge balance shifted due to protonation of the PAaH block. After collapse of the PIC vesicles, P20A190 formed micelles composed of protonated PAaH core and PMPC shells, while P20M167 was released as unimers. PIC vesicles can encapsulate hydrophilic nonionic guest molecules into their hollow core. Under acidic conditions, the PIC vesicles can release the guest molecules and P20M167. The cationic P20M167 can break the lysosome membrane to efficiently release the guest molecules from the lysosomes to the cytoplasm
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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| 700 |
1 |
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|a Nakai, Keita
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Ahmed, Sana
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Matsumura, Kazuaki
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Ishihara, Kazuhiko
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Yusa, Shin-Ichi
|e verfasserin
|4 aut
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| 773 |
0 |
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|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1992
|g 35(2019), 5 vom: 05. Feb., Seite 1249-1256
|w (DE-627)NLM098181009
|x 1520-5827
|7 nnns
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| 773 |
1 |
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|g volume:35
|g year:2019
|g number:5
|g day:05
|g month:02
|g pages:1249-1256
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|u http://dx.doi.org/10.1021/acs.langmuir.8b00632
|3 Volltext
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