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231225s2018 xx |||||o 00| ||eng c |
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|a 10.2144/btn-2018-2000
|2 doi
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|a pubmed25n0943.xml
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|a eng
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| 100 |
1 |
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|a Conte, Jillian
|e verfasserin
|4 aut
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| 245 |
1 |
0 |
|a Using synthetic oligonucleotides as standards in probe-based qPCR
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|c 2018
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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| 500 |
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|a Date Completed 24.06.2019
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|a Date Revised 24.06.2019
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|a published: Print
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|a Citation Status MEDLINE
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|a Real-time PCR (qPCR) is widely used in the life sciences. For quantifying DNA, a standard curve is required. Common methods for standard development are time consuming, costly, necessitate a specific skill set, and pose a contamination risk. Using a targeted synthetic oligonucleotide, such as a gBlocks® Gene Fragment, overcomes these drawbacks and provides researchers an accurate and quick solution to standard development. Here, we demonstrate that using a gBlocks fragment as a standard provides comparable sensitivity, reliability, and assay performance to a purified amplicon standard
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a DNA standard
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|a gBlocks®
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|a qPCR
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4 |
|a synthetic oligonucleotide
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|a DNA Primers
|2 NLM
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| 650 |
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7 |
|a DNA Probes
|2 NLM
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| 650 |
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7 |
|a Oligonucleotides
|2 NLM
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| 650 |
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|a DNA
|2 NLM
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| 650 |
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|a 9007-49-2
|2 NLM
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| 700 |
1 |
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|a Potoczniak, Margret J
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Tobe, Shanan S
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t BioTechniques
|d 1993
|g 64(2018), 4 vom: 20. Apr., Seite 177-179
|w (DE-627)NLM012627046
|x 1940-9818
|7 nnns
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| 773 |
1 |
8 |
|g volume:64
|g year:2018
|g number:4
|g day:20
|g month:04
|g pages:177-179
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4 |
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|u http://dx.doi.org/10.2144/btn-2018-2000
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|d 64
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|h 177-179
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