Using synthetic oligonucleotides as standards in probe-based qPCR

Real-time PCR (qPCR) is widely used in the life sciences. For quantifying DNA, a standard curve is required. Common methods for standard development are time consuming, costly, necessitate a specific skill set, and pose a contamination risk. Using a targeted synthetic oligonucleotide, such as a gBlo...

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Bibliographische Detailangaben

Veröffentlicht in:	BioTechniques. - 1993. - 64(2018), 4 vom: 20. Apr., Seite 177-179
1. Verfasser:	Conte, Jillian (VerfasserIn)
Weitere Verfasser:	Potoczniak, Margret J, Tobe, Shanan S
Format:	Online-Aufsatz
Sprache:	English
Veröffentlicht:	2018
Zugriff auf das übergeordnete Werk:	BioTechniques
Schlagworte:	Journal Article Research Support, Non-U.S. Gov't DNA standard gBlocks® qPCR synthetic oligonucleotide DNA Primers DNA Probes Oligonucleotides DNA 9007-49-2