Identification of an algal xylan synthase indicates that there is functional orthology between algal and plant cell wall biosynthesis

Identification of an algal xylan synthase indicates that there is functional orthology between algal and plant cell wall biosynthesis

© 2018 The Authors. New Phytologist © 2018 New Phytologist Trust.

Bibliographische Detailangaben
Veröffentlicht in:The New phytologist. - 1979. - 218(2018), 3 vom: 26. Mai, Seite 1049-1060
1. Verfasser: Jensen, Jacob Krüger (VerfasserIn)
Weitere Verfasser: Busse-Wicher, Marta, Poulsen, Christian Peter, Fangel, Jonatan Ulrik, Smith, Peter James, Yang, Jeong-Yeh, Peña, Maria-Jesus, Dinesen, Malene Hessellund, Martens, Helle Juel, Melkonian, Michael, Wong, Gane Ka-Shu, Moremen, Kelley W, Wilkerson, Curtis Gene, Scheller, Henrik Vibe, Dupree, Paul, Ulvskov, Peter, Urbanowicz, Breeanna Rae, Harholt, Jesper
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2018
Zugriff auf das übergeordnete Werk:The New phytologist
Schlagworte:Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Klebsormidium flaccidum Klebsormidium nitens IRX10 XYS1 biosynthesis cell wall mehr... evolution xylan Pentosyltransferases EC 2.4.2.- 1,4-beta-D-xylan synthase EC 2.4.2.24
Beschreibung
Zusammenfassung:© 2018 The Authors. New Phytologist © 2018 New Phytologist Trust.
Insights into the evolution of plant cell walls have important implications for comprehending these diverse and abundant biological structures. In order to understand the evolving structure-function relationships of the plant cell wall, it is imperative to trace the origin of its different components. The present study is focused on plant 1,4-β-xylan, tracing its evolutionary origin by genome and transcriptome mining followed by phylogenetic analysis, utilizing a large selection of plants and algae. It substantiates the findings by heterologous expression and biochemical characterization of a charophyte alga xylan synthase. Of the 12 known gene classes involved in 1,4-β-xylan formation, XYS1/IRX10 in plants, IRX7, IRX8, IRX9, IRX14 and GUX occurred for the first time in charophyte algae. An XYS1/IRX10 ortholog from Klebsormidium flaccidum, designated K. flaccidumXYLAN SYNTHASE-1 (KfXYS1), possesses 1,4-β-xylan synthase activity, and 1,4-β-xylan occurs in the K. flaccidum cell wall. These data suggest that plant 1,4-β-xylan originated in charophytes and shed light on the origin of one of the key cell wall innovations to occur in charophyte algae, facilitating terrestrialization and emergence of polysaccharide-based plant cell walls
Beschreibung:Date Completed 01.10.2019
Date Revised 30.09.2020
published: Print-Electronic
Citation Status MEDLINE
ISSN:1469-8137
DOI:10.1111/nph.15050