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20231225024826.0 |
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231225s2018 xx |||||o 00| ||eng c |
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|a 10.1002/adma.201705443
|2 doi
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|a pubmed24n0934.xml
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|a (DE-627)NLM280214502
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|a (NLM)29359821
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Zhang, Rongzhen
|e verfasserin
|4 aut
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| 245 |
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|a Biofunctionalized "Kiwifruit-Assembly" of Oxidoreductases in Mesoporous ZnO/Carbon Nanoparticles for Efficient Asymmetric Catalysis
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|c 2018
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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| 500 |
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|a Date Completed 07.03.2019
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|a Date Revised 21.03.2022
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a © 2018 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
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|a A mesoporous ZnO/carbon composite is designed for coimmobilization of two oxidoreductases involving a novel "kiwifruit-assembly" pattern. The coimmobilization of (S)-carbonyl reductase II-glucose dehydrogenase on nanoparticles (SCRII-GDHnano ) exhibits 40-50% higher specific activity than the free enzyme and significantly improves stabilities of enzymes to heat, pH and solvents. It performs asymmetric catalysis of 75 × 10-3 m substrate with a perfect yield of 100% and an excellent enantioselectivity of 99.9% within 1 h. SCRII-GDHnano gives an over 72% yield and 99.9% enantioselectivity after it is reused for ten times. Even with a highly concentrated (400 × 10-3 m) substrate, it shows about 60% yield and 99.9% enantioselectivity within 4 h. SCRII-GDHnano presents 4.5-8.0-fold higher productivity in 2.0-8.0-fold shorter reaction time than the free enzyme. This work provides a general, facile, and unique approach for the immobilization of two oxidoreductases and gives high catalytic efficiency, long-term and good recycling stabilities by triggering radical proton-coupled electron transfer
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|a Journal Article
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|a asymmetric catalysis
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|a coencapsulation of oxidoreductases
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|a mesoporous ZnO/carbon nanoparticles
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|a radical proton-coupled electron transfer
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|a recycling stability
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|a Carbon
|2 NLM
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|a 7440-44-0
|2 NLM
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|a Oxidoreductases
|2 NLM
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|a EC 1.-
|2 NLM
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|a Zinc Oxide
|2 NLM
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| 650 |
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|a SOI2LOH54Z
|2 NLM
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| 700 |
1 |
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|a Jiang, Jiawei
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Zhou, Junping
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Xu, Yan
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Xiao, Rong
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Xia, Xinhui
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Rao, Zhiming
|e verfasserin
|4 aut
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| 773 |
0 |
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|i Enthalten in
|t Advanced materials (Deerfield Beach, Fla.)
|d 1998
|g 30(2018), 11 vom: 07. März
|w (DE-627)NLM098206397
|x 1521-4095
|7 nnns
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| 773 |
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|g volume:30
|g year:2018
|g number:11
|g day:07
|g month:03
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|u http://dx.doi.org/10.1002/adma.201705443
|3 Volltext
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