Biofunctionalized "Kiwifruit-Assembly" of Oxidoreductases in Mesoporous ZnO/Carbon Nanoparticles for Efficient Asymmetric Catalysis
© 2018 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
| Veröffentlicht in: | Advanced materials (Deerfield Beach, Fla.). - 1998. - 30(2018), 11 vom: 07. März |
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| Weitere Verfasser: | , , , , , |
| Format: | Online-Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
2018
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| Zugriff auf das übergeordnete Werk: | Advanced materials (Deerfield Beach, Fla.) |
| Schlagworte: | Journal Article asymmetric catalysis coencapsulation of oxidoreductases mesoporous ZnO/carbon nanoparticles radical proton-coupled electron transfer recycling stability Carbon 7440-44-0 Oxidoreductases EC 1.- mehr... |
| Zusammenfassung: | © 2018 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. A mesoporous ZnO/carbon composite is designed for coimmobilization of two oxidoreductases involving a novel "kiwifruit-assembly" pattern. The coimmobilization of (S)-carbonyl reductase II-glucose dehydrogenase on nanoparticles (SCRII-GDHnano ) exhibits 40-50% higher specific activity than the free enzyme and significantly improves stabilities of enzymes to heat, pH and solvents. It performs asymmetric catalysis of 75 × 10-3 m substrate with a perfect yield of 100% and an excellent enantioselectivity of 99.9% within 1 h. SCRII-GDHnano gives an over 72% yield and 99.9% enantioselectivity after it is reused for ten times. Even with a highly concentrated (400 × 10-3 m) substrate, it shows about 60% yield and 99.9% enantioselectivity within 4 h. SCRII-GDHnano presents 4.5-8.0-fold higher productivity in 2.0-8.0-fold shorter reaction time than the free enzyme. This work provides a general, facile, and unique approach for the immobilization of two oxidoreductases and gives high catalytic efficiency, long-term and good recycling stabilities by triggering radical proton-coupled electron transfer |
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| Beschreibung: | Date Completed 07.03.2019 Date Revised 21.03.2022 published: Print-Electronic Citation Status MEDLINE |
| ISSN: | 1521-4095 |
| DOI: | 10.1002/adma.201705443 |