Arabidopsis ACYL-COA-BINDING PROTEIN1 interacts with STEROL C4-METHYL OXIDASE1-2 to modulate gene expression of homeodomain-leucine zipper IV transcription factors

Bibliographische Detailangaben
Veröffentlicht in:	The New phytologist. - 1979. - 218(2018), 1 vom: 30. Apr., Seite 183-200
1. Verfasser:	Lung, Shiu-Cheung (VerfasserIn)
Weitere Verfasser:	Liao, Pan, Yeung, Edward C, Hsiao, An-Shan, Xue, Yan, Chye, Mee-Len
Format:	Online-Aufsatz
Sprache:	English
Veröffentlicht:	2018
Zugriff auf das übergeordnete Werk:	The New phytologist
Schlagworte:	Journal Article Research Support, Non-U.S. Gov't GLABRA2 embryogenesis fatty acid lipid signaling protein interactor second messenger sterol trichome mehr... ACBP1 protein, Arabidopsis Arabidopsis Proteins Basic-Leucine Zipper Transcription Factors Carrier Proteins Fatty Acids Homeodomain Proteins Multiprotein Complexes Sterols AT4G22756 protein, Arabidopsis EC 1.- Mixed Function Oxygenases

Beschreibung
Zusammenfassung:	© 2018 The Authors. New Phytologist © 2018 New Phytologist Trust. Fatty acids (FAs) and sterols constitute building blocks of eukaryotic membranes and lipid signals. Co-regulation of FA and sterol synthesis is mediated by sterol regulatory element-binding proteins in animals but remains elusive in plants. We reported recently that Arabidopsis ACYL-COA-BINDING PROTEIN1 (ACBP1) modulates sterol synthesis via protein-protein interaction with STEROL C4-METHYL OXIDASE1-1 (SMO1-1). Herein, ACBP1 was demonstrated to co-express and interact with SMO1-2 by yeast two-hybrid, co-localization, pull-down, co-immunoprecipitation and β-glucuronidase assays. SMO1-2 silenced in acbp1 was used in phenotyping, GC-MS and expression profiling. ACBP1 co-expressed with SMO1-2 in embryo sacs, pollen and trichomes, corroborating with cooperative tissue-specific functions unseen with SMO1-1. SMO1-2 silencing in acbp1 impaired seed development, male and female gamete transmission, and pollen function. Genes encoding homeodomain-leucine zipper IV transcription factors (HDG5, HDG10, HDG11 and GLABRA2), which potentially bind phospholipids/sterols, were transcribed aberrantly. GLABRA2 targets (MYB23, MUM4 and PLDα1) were misregulated, causing glabra2-resembling trichome, seed coat mucilage and oil-accumulating phenotypes. Together with altered sterol and FA compositions upon ACBP1 mutation and/or SMO1-2 silencing, ACBP1-SMO1 interaction appears to mediate homeostatic co-regulation of FAs and sterols, which serve as lipid modulators for gene expression of homeodomain-leucine zipper IV transcription factors
Beschreibung:	Date Completed 27.09.2019 Date Revised 30.09.2020 published: Print-Electronic Citation Status MEDLINE
ISSN:	1469-8137
DOI:	10.1111/nph.14965