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231225s2017 xx |||||o 00| ||eng c |
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|a 10.2166/wst.2017.510
|2 doi
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|a pubmed24n0930.xml
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|a (DE-627)NLM279007299
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|a (NLM)29236024
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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|a Yang, Yunlong
|e verfasserin
|4 aut
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|a Heterotrophic nitrogen removal in Bacillus sp. K5
|b involvement of a novel hydroxylamine oxidase
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|c 2017
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
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|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 03.05.2018
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|a Date Revised 02.12.2018
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|a published: Print
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|a Citation Status MEDLINE
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|a An aerobic denitrifying bacterium isolated from a bio-trickling filter treating NOx, Bacillus sp. K5, is able to convert ammonium to nitrite, in which hydroxylamine oxidase (HAO) plays a critical role. In the present study, the performance for simultaneous nitrification and denitrification was investigated with batch experiments and an HAO was purified by an anion-exchange and gel-filtration chromatography from strain K5. The purified HAO's molecular mass was determined by SDS-PAGE and its activity by measuring the change in the concentration of ferricyanide, the electron acceptor. Results showed that as much as 87.8 mg L-1 ammonium-N was removed without nitrite accumulation within 24 hours in the sodium citrate medium at C/N of 15. The HAO isolated from the strain K5 was approximately 71 KDa. With hydroxylamine (NH2OH) as a substrate and potassium ferricyanide as an electron acceptor, the enzyme was capable of oxidizing NH2OH to nitrite in vitro when the pH varied from 7 to 9 and temperature ranged from 25 °C to 40 °C. This is the first time that an HAO has been purified from the Bacillus genus, and the findings revealed that it is distinctive in its molecular mass and enzyme properties
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|a Journal Article
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|a Ammonium Compounds
|2 NLM
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|a Nitrites
|2 NLM
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|a Hydroxylamine
|2 NLM
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|a 2FP81O2L9Z
|2 NLM
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|a Oxidoreductases
|2 NLM
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|a EC 1.-
|2 NLM
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|a hydroxylamine dehydrogenase
|2 NLM
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|a EC 1.7.2.6
|2 NLM
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|a Nitrogen
|2 NLM
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|a N762921K75
|2 NLM
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1 |
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|a Lin, Ershu
|e verfasserin
|4 aut
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|a Huang, Shaobin
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Water science and technology : a journal of the International Association on Water Pollution Research
|d 1986
|g 76(2017), 11-12 vom: 09. Dez., Seite 3461-3467
|w (DE-627)NLM098149431
|x 0273-1223
|7 nnns
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| 773 |
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|g volume:76
|g year:2017
|g number:11-12
|g day:09
|g month:12
|g pages:3461-3467
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|u http://dx.doi.org/10.2166/wst.2017.510
|3 Volltext
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