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231225s2018 xx |||||o 00| ||eng c |
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|a 10.1021/acs.langmuir.7b03310
|2 doi
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|a pubmed24n0929.xml
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|a DE-627
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|a eng
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|a Deodhar, Gauri V
|e verfasserin
|4 aut
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|a Conserved Activity of Reassociated Homotetrameric Protein Subunits Released from Mesoporous Silica Nanoparticles
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|c 2018
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
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|2 rdamedia
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|a ƒa Online-Ressource
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|2 rdacarrier
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|a Date Completed 11.09.2018
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|a Date Revised 11.09.2018
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Mesoporous silica nanoparticles (MSN) with enlarged pores were prepared and characterized, and reversibly dissociated subunits of concanavalin A were entrapped in the mesopores, as shown by multiple biochemical and material characterizations. When loaded in the MSN, we demonstrated protein stability from proteases and, upon release, the subunits reassociated into active proteins shown through mannose binding and o-phthalaldehyde fluorescence. We have demonstrated a versatile and facile method to load homomeric proteins into MSN with potential applications in enhancing the delivery of large therapeutic proteins
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Drug Carriers
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|a Protein Subunits
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|a Concanavalin A
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|a 11028-71-0
|2 NLM
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|a Silicon Dioxide
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|a 7631-86-9
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|a Peptide Hydrolases
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|a EC 3.4.-
|2 NLM
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|a Adams, Marisa L
|e verfasserin
|4 aut
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|a Joardar, Sutapa
|e verfasserin
|4 aut
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|a Joglekar, Madhura
|e verfasserin
|4 aut
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|a Davidson, Malcolm
|e verfasserin
|4 aut
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|a Smith, William C
|e verfasserin
|4 aut
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|a Mettler, Madelyn
|e verfasserin
|4 aut
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|a Toler, Sydney A
|e verfasserin
|4 aut
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|a Davies, Fiona K
|e verfasserin
|4 aut
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|a Williams, S Kim R
|e verfasserin
|4 aut
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|a Trewyn, Brian G
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1992
|g 34(2018), 1 vom: 09. Jan., Seite 228-233
|w (DE-627)NLM098181009
|x 1520-5827
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|g volume:34
|g year:2018
|g number:1
|g day:09
|g month:01
|g pages:228-233
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|u http://dx.doi.org/10.1021/acs.langmuir.7b03310
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