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cr uuu---uuuuu |
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231225s2018 xx |||||o 00| ||eng c |
| 024 |
7 |
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|a 10.1111/nph.14854
|2 doi
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| 028 |
5 |
2 |
|a pubmed24n0925.xml
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|a (DE-627)NLM277518059
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|a (NLM)29084344
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|a DE-627
|b ger
|c DE-627
|e rakwb
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| 041 |
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|a eng
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| 100 |
1 |
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|a Wang, Yafei
|e verfasserin
|4 aut
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| 245 |
1 |
0 |
|a VirD5 is required for efficient Agrobacterium infection and interacts with Arabidopsis VIP2
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| 264 |
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1 |
|c 2018
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| 336 |
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|a Text
|b txt
|2 rdacontent
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| 337 |
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|a ƒaComputermedien
|b c
|2 rdamedia
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| 338 |
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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| 500 |
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|a Date Completed 12.09.2019
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| 500 |
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|a Date Revised 30.09.2020
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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| 520 |
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|a © 2017 The Authors. New Phytologist © 2017 New Phytologist Trust.
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|a During Agrobacterium (Agrobacterium tumefaciens) infection, the translocated virulence proteins (VirD2, VirE2, VirE3, VirF and VirD5) play crucial roles. It is thought that, through protein-protein interactions, Agrobacterium uses and abuses host plant factors and systems to facilitate its infection. Although some molecular functions have been revealed, the roles of VirD5 still need to be further elucidated. Here, plant transformation and tumorigenesis mediated by genetically modified Agrobacterium strains were performed to examine VirD5 roles. In addition, protein-protein interaction-associated molecular and biochemistry technologies were used to reveal and elucidate VirD5 interaction with Arabidopsis VirE2 interacting protein 2 (VIP2). Our results showed that deleting virD5 from Agrobacterium reduced its tumor formation ability and stable transformation efficiency but did not affect the transient transformation efficiency. We also found that VirD5 can interact with Arabidopsis VIP2. Further experiments demonstrated that VirD5 can affect VIP2 binding to cap-binding proteins (CBP20 and CBP80). The tumorigenesis efficiency for cbp80 mutant was not significantly changed, but that for cbp20, cbp20cbp80 mutants were significantly increased. This work demonstrates experimentally that VirD5 is required for efficient Agrobacterium infection and may promote this process by competitive interaction with Arabidopsis VIP2. CBP20 is involved in the Agrobacterium infection process and its effect can be synergistically enhanced by CBP80
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| 650 |
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4 |
|a Journal Article
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| 650 |
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4 |
|a Research Support, Non-U.S. Gov't
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| 650 |
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4 |
|a Agrobacterium tumefaciens
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| 650 |
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4 |
|a Arabidopsis
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| 650 |
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4 |
|a VIP2
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| 650 |
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4 |
|a VirD5
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| 650 |
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4 |
|a cap-binding proteins (CBPs)
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| 650 |
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4 |
|a plant transformation
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| 650 |
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4 |
|a plant tumor
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| 650 |
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7 |
|a Arabidopsis Proteins
|2 NLM
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| 650 |
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7 |
|a Transcription Factors, General
|2 NLM
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| 650 |
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7 |
|a VIP2 protein, Arabidopsis
|2 NLM
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| 650 |
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7 |
|a Virulence Factors
|2 NLM
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| 700 |
1 |
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|a Zhang, Shaojuan
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Huang, Fei
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Zhou, Xu
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Chen, Zhuo
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Peng, Wei
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Luo, Meizhong
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t The New phytologist
|d 1979
|g 217(2018), 2 vom: 30. Jan., Seite 726-738
|w (DE-627)NLM09818248X
|x 1469-8137
|7 nnns
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| 773 |
1 |
8 |
|g volume:217
|g year:2018
|g number:2
|g day:30
|g month:01
|g pages:726-738
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| 856 |
4 |
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|u http://dx.doi.org/10.1111/nph.14854
|3 Volltext
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|a GBV_USEFLAG_A
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|a SYSFLAG_A
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|a GBV_NLM
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|a GBV_ILN_350
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|a AR
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| 952 |
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|d 217
|j 2018
|e 2
|b 30
|c 01
|h 726-738
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