Biotoxicity of Cry1Ab protein on wolf spider Pardosa pseudoannulata

In this research, we carried out a tritrophic bioassay to assess the potential effect of Cry1Ab-expressing rice on the foraging behavior of the common wolf spider Pardosa pseudoannulata and its underlying molecular mechanism. Results indicated the Bt-containing spiders expressed a higher foraging ra...

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Veröffentlicht in:Ecotoxicology (London, England). - 1992. - 26(2017), 10 vom: 17. Dez., Seite 1336-1343
1. Verfasser: Wang, Juan (VerfasserIn)
Weitere Verfasser: Peng, Yuande, Yang, Huilin, Yan, Zhenzhen, Li, Qinjin, Shi, Yixue, Xie, Chunliang, Liang, Yunshan, Wang, Zhi, Song, Qisheng
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2017
Zugriff auf das übergeordnete Werk:Ecotoxicology (London, England)
Schlagworte:Journal Article CNS Cry1Ab P. pseudoannulata behavior transcriptome Bacillus thuringiensis Toxins Bacterial Proteins Endotoxins Hemolysin Proteins insecticidal crystal protein, Bacillus Thuringiensis
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520 |a In this research, we carried out a tritrophic bioassay to assess the potential effect of Cry1Ab-expressing rice on the foraging behavior of the common wolf spider Pardosa pseudoannulata and its underlying molecular mechanism. Results indicated the Bt-containing spiders expressed a higher foraging range when compared to controls. The high throughput de novo transcriptome sequencing was further carried out for central nervous system (CNS) of P. pseudoannulata with and without Cry1Ab intake. We obtained 120, 985 unigenes with a mean length of 529.73 bp. Functional analysis of differentially expressed genes (DEGs) showed the expression levels of genes related to energy metabolism were changed in response to Cry1Ab, which may contribute to a more active foraging behavior. In addition, some DEGs also have a function related to metal ion binding, implying a potential influence on metal ions-dependent reactions. This may be associated with Cry1Ab resistance mechanism in the spider 
650 4 |a Journal Article 
650 4 |a CNS 
650 4 |a Cry1Ab 
650 4 |a P. pseudoannulata 
650 4 |a behavior 
650 4 |a transcriptome 
650 7 |a Bacillus thuringiensis Toxins  |2 NLM 
650 7 |a Bacterial Proteins  |2 NLM 
650 7 |a Endotoxins  |2 NLM 
650 7 |a Hemolysin Proteins  |2 NLM 
650 7 |a insecticidal crystal protein, Bacillus Thuringiensis  |2 NLM 
700 1 |a Peng, Yuande  |e verfasserin  |4 aut 
700 1 |a Yang, Huilin  |e verfasserin  |4 aut 
700 1 |a Yan, Zhenzhen  |e verfasserin  |4 aut 
700 1 |a Li, Qinjin  |e verfasserin  |4 aut 
700 1 |a Shi, Yixue  |e verfasserin  |4 aut 
700 1 |a Xie, Chunliang  |e verfasserin  |4 aut 
700 1 |a Liang, Yunshan  |e verfasserin  |4 aut 
700 1 |a Wang, Zhi  |e verfasserin  |4 aut 
700 1 |a Song, Qisheng  |e verfasserin  |4 aut 
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773 1 8 |g volume:26  |g year:2017  |g number:10  |g day:17  |g month:12  |g pages:1336-1343 
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