Biotoxicity of Cry1Ab protein on wolf spider Pardosa pseudoannulata
In this research, we carried out a tritrophic bioassay to assess the potential effect of Cry1Ab-expressing rice on the foraging behavior of the common wolf spider Pardosa pseudoannulata and its underlying molecular mechanism. Results indicated the Bt-containing spiders expressed a higher foraging ra...
| Veröffentlicht in: | Ecotoxicology (London, England). - 1992. - 26(2017), 10 vom: 17. Dez., Seite 1336-1343 |
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| 1. Verfasser: | |
| Weitere Verfasser: | , , , , , , , , |
| Format: | Online-Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
2017
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| Zugriff auf das übergeordnete Werk: | Ecotoxicology (London, England) |
| Schlagworte: | Journal Article CNS Cry1Ab P. pseudoannulata behavior transcriptome Bacillus thuringiensis Toxins Bacterial Proteins Endotoxins Hemolysin Proteins |
| Zusammenfassung: | In this research, we carried out a tritrophic bioassay to assess the potential effect of Cry1Ab-expressing rice on the foraging behavior of the common wolf spider Pardosa pseudoannulata and its underlying molecular mechanism. Results indicated the Bt-containing spiders expressed a higher foraging range when compared to controls. The high throughput de novo transcriptome sequencing was further carried out for central nervous system (CNS) of P. pseudoannulata with and without Cry1Ab intake. We obtained 120, 985 unigenes with a mean length of 529.73 bp. Functional analysis of differentially expressed genes (DEGs) showed the expression levels of genes related to energy metabolism were changed in response to Cry1Ab, which may contribute to a more active foraging behavior. In addition, some DEGs also have a function related to metal ion binding, implying a potential influence on metal ions-dependent reactions. This may be associated with Cry1Ab resistance mechanism in the spider |
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| Beschreibung: | Date Completed 07.12.2017 Date Revised 09.12.2020 published: Print-Electronic Citation Status MEDLINE |
| ISSN: | 1573-3017 |
| DOI: | 10.1007/s10646-017-1858-4 |