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231225s2017 xx |||||o 00| ||eng c |
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|a 10.1111/nph.14713
|2 doi
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|a pubmed24n0913.xml
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|a (DE-627)NLM274120658
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|a (NLM)28737213
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Schulze, Stefan
|e verfasserin
|4 aut
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| 245 |
1 |
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|a Identification of methylated GnTI-dependent N-glycans in Botryococcus brauni
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| 264 |
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|c 2017
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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| 338 |
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 15.05.2018
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|a Date Revised 30.03.2022
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a © 2017 The Authors. New Phytologist © 2017 New Phytologist Trust.
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|a In contrast to mammals and vascular plants, microalgae show a high diversity in the N-glycan structures of complex N-glycoproteins. Although homologues for β1,2-N-acetylglucosaminyltransferase I (GnTI), a key enzyme in the formation of complex N-glycans, have been identified in several algal species, GnTI-dependent N-glycans have not been detected so far. We have performed an N-glycoproteomic analysis of the hydrocarbon oils accumulating green microalgae Botryococcus braunii. Thereby, the analysis of intact N-glycopeptides allowed the determination of N-glycan compositions. Furthermore, insights into the role of N-glycosylation in B. braunii were gained from functional annotation of the identified N-glycoproteins. In total, 517 unique N-glycosylated peptides have been identified, including intact N-glycopeptides that harbored N-acetylhexosamine (HexNAc) at the nonreducing end. Surprisingly, these GnTI-dependent N-glycans were also found to be modified with (di)methylated hexose. The identification of GnTI-dependent N-glycans in combination with N-glycan methylation in B. braunii revealed an uncommon type of N-glycan processing in this microalgae
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|a Journal Article
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4 |
|a Botryococcus braunii
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|a N-glycosylation
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|a gene ontology annotation
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|a mass spectrometry
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|a post-translational modification
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|a Glycopeptides
|2 NLM
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|a Glycoproteins
|2 NLM
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| 650 |
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|a Oxygen Isotopes
|2 NLM
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| 650 |
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|a Polysaccharides
|2 NLM
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| 650 |
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|a N-Acetylglucosaminyltransferases
|2 NLM
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| 650 |
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7 |
|a EC 2.4.1.-
|2 NLM
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| 650 |
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7 |
|a UDP-GlcNAc alpha-D-mannoside beta-1,2-N-acetylglucosaminyltransferase
|2 NLM
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| 650 |
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7 |
|a EC 2.4.1.-
|2 NLM
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| 700 |
1 |
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|a Urzica, Eugen
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Reijnders, Maarten J M F
|e verfasserin
|4 aut
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| 700 |
1 |
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|a van de Geest, Henri
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Warris, Sven
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Bakker, Linda V
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Fufezan, Christian
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Martins Dos Santos, Vitor A P
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Schaap, Peter J
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Peters, Sander A
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Hippler, Michael
|e verfasserin
|4 aut
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| 773 |
0 |
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|i Enthalten in
|t The New phytologist
|d 1979
|g 215(2017), 4 vom: 02. Sept., Seite 1361-1369
|w (DE-627)NLM09818248X
|x 1469-8137
|7 nnns
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| 773 |
1 |
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|g volume:215
|g year:2017
|g number:4
|g day:02
|g month:09
|g pages:1361-1369
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|u http://dx.doi.org/10.1111/nph.14713
|3 Volltext
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|d 215
|j 2017
|e 4
|b 02
|c 09
|h 1361-1369
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