Identification of methylated GnTI-dependent N-glycans in Botryococcus brauni

Identification of methylated GnTI-dependent N-glycans in Botryococcus brauni

© 2017 The Authors. New Phytologist © 2017 New Phytologist Trust.

Bibliographische Detailangaben
Veröffentlicht in:The New phytologist. - 1979. - 215(2017), 4 vom: 02. Sept., Seite 1361-1369
1. Verfasser: Schulze, Stefan (VerfasserIn)
Weitere Verfasser: Urzica, Eugen, Reijnders, Maarten J M F, van de Geest, Henri, Warris, Sven, Bakker, Linda V, Fufezan, Christian, Martins Dos Santos, Vitor A P, Schaap, Peter J, Peters, Sander A, Hippler, Michael
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2017
Zugriff auf das übergeordnete Werk:The New phytologist
Schlagworte:Journal Article Botryococcus braunii N-glycosylation gene ontology annotation mass spectrometry post-translational modification Glycopeptides Glycoproteins Oxygen Isotopes Polysaccharides mehr... N-Acetylglucosaminyltransferases EC 2.4.1.- UDP-GlcNAc alpha-D-mannoside beta-1,2-N-acetylglucosaminyltransferase
Beschreibung
Zusammenfassung:© 2017 The Authors. New Phytologist © 2017 New Phytologist Trust.
In contrast to mammals and vascular plants, microalgae show a high diversity in the N-glycan structures of complex N-glycoproteins. Although homologues for β1,2-N-acetylglucosaminyltransferase I (GnTI), a key enzyme in the formation of complex N-glycans, have been identified in several algal species, GnTI-dependent N-glycans have not been detected so far. We have performed an N-glycoproteomic analysis of the hydrocarbon oils accumulating green microalgae Botryococcus braunii. Thereby, the analysis of intact N-glycopeptides allowed the determination of N-glycan compositions. Furthermore, insights into the role of N-glycosylation in B. braunii were gained from functional annotation of the identified N-glycoproteins. In total, 517 unique N-glycosylated peptides have been identified, including intact N-glycopeptides that harbored N-acetylhexosamine (HexNAc) at the nonreducing end. Surprisingly, these GnTI-dependent N-glycans were also found to be modified with (di)methylated hexose. The identification of GnTI-dependent N-glycans in combination with N-glycan methylation in B. braunii revealed an uncommon type of N-glycan processing in this microalgae
Beschreibung:Date Completed 15.05.2018
Date Revised 30.03.2022
published: Print-Electronic
Citation Status MEDLINE
ISSN:1469-8137
DOI:10.1111/nph.14713