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231225s2017 xx |||||o 00| ||eng c |
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|a 10.1021/acs.langmuir.7b01959
|2 doi
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|a pubmed24n0911.xml
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|a (DE-627)NLM273347926
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|a (NLM)28657327
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Neveshkin, Alexander
|e verfasserin
|4 aut
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| 245 |
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|a Polydopamine Coating To Stabilize a Free-Standing Lipid Bilayer for Channel Sensing
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|c 2017
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 16.01.2019
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|a Date Revised 16.01.2019
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a An appropriate method to study the function of membrane channels is to insert them into free-standing lipid bilayers and to record the ion conductance across the membrane. The insulating property of a free-standing lipid bilayer versus the single-channel conductivity provides sufficient sensitivity to detect minor changes in the pathway of ions along the channel. A potential application is to use membrane channels as label-free sensors for molecules, with DNA sequencing as its most prominent application. However, the inherent instability of free-standing bilayers limits broader use as a biosensor. Here we report on a possible stabilization of free-standing lipid bilayers using polydopamine deposition from dopamine-containing solutions in the presence of an oxidant. This stabilization treatment can be initiated after protein reconstitution and is compatible with most reconstitution protocols
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Indoles
|2 NLM
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|a Ion Channels
|2 NLM
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|a Lipid Bilayers
|2 NLM
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| 650 |
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|a Polymers
|2 NLM
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| 650 |
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|a polydopamine
|2 NLM
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| 700 |
1 |
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|a Citak, Funda
|e verfasserin
|4 aut
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|a Ball, Vincent
|e verfasserin
|4 aut
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|a Winterhalter, Mathias
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1992
|g 33(2017), 29 vom: 25. Juli, Seite 7256-7262
|w (DE-627)NLM098181009
|x 1520-5827
|7 nnns
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| 773 |
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|g volume:33
|g year:2017
|g number:29
|g day:25
|g month:07
|g pages:7256-7262
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|u http://dx.doi.org/10.1021/acs.langmuir.7b01959
|3 Volltext
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