|
|
|
|
| LEADER |
01000naa a22002652 4500 |
| 001 |
NLM273282573 |
| 003 |
DE-627 |
| 005 |
20231225000843.0 |
| 007 |
cr uuu---uuuuu |
| 008 |
231225s2017 xx |||||o 00| ||eng c |
| 024 |
7 |
|
|a 10.1111/jpy.12556
|2 doi
|
| 028 |
5 |
2 |
|a pubmed24n0910.xml
|
| 035 |
|
|
|a (DE-627)NLM273282573
|
| 035 |
|
|
|a (NLM)28650572
|
| 040 |
|
|
|a DE-627
|b ger
|c DE-627
|e rakwb
|
| 041 |
|
|
|a eng
|
| 100 |
1 |
|
|a Guo, Yang
|e verfasserin
|4 aut
|
| 245 |
1 |
0 |
|a Cloning and characterization of nitrate reductase gene in Ulva prolifera (Ulvophyceae, Chlorophyta)
|
| 264 |
|
1 |
|c 2017
|
| 336 |
|
|
|a Text
|b txt
|2 rdacontent
|
| 337 |
|
|
|a ƒaComputermedien
|b c
|2 rdamedia
|
| 338 |
|
|
|a ƒa Online-Ressource
|b cr
|2 rdacarrier
|
| 500 |
|
|
|a Date Completed 14.05.2018
|
| 500 |
|
|
|a Date Revised 03.12.2018
|
| 500 |
|
|
|a published: Print-Electronic
|
| 500 |
|
|
|a GENBANK: SRX151604, XP_001696697, KY438973, XP_002955156, AAL79356, ACF22999, NP_177899, XM_001696645, CVU39931, XM_005844731, XM_005647773, X64136, AB232049, NM_106425, XM_015794814
|
| 500 |
|
|
|a Citation Status MEDLINE
|
| 520 |
|
|
|a © 2017 Phycological Society of America.
|
| 520 |
|
|
|a Ulva spp. dominates green tides around the world, which are occurring at an accelerated rate. The competitive nitrogen assimilation efficiency in Ulva is suggested to result in ecological success against other seaweeds. However, molecular characterization of genes involved in nitrogen assimilation has not been conducted. Here, we describe the identification of the nitrate reductase (NR) gene from a green seaweed Ulva prolifera, an alga which is responsible for the world's largest green tide in the Yellow Sea. Using rapid amplification of cDNA ends and genome walking, the NR gene from U. prolifera (UpNR) was cloned, which consisted of six introns and seven exons encoding 863 amino acids. According to sequence alignment, the NR in U. prolifera was shown to possess all five essential domains and 21 key invariant residues in plant NRs. The GC content of third codon position of UpNR (82.75%) was as high as those of green microalgae, and the intron number supported a potential loss issue from green microalga to land plant. Real-time quantitative PCR results showed that UpNR transcript level was induced by nitrate and repressed by ammonium, which could not be removed by addition of extra nitrate, indicating that U. prolifera preferred ammonium to nitrate. Urea would not repress NR transcription by itself, while it weakened the induction effect of nitrate, implying it possibly inhibited nitrate uptake rather than nitrate reduction. These results suggest the use of UpNR as a gene-sensor to probe the N assimilation process in green tides caused by Ulva
|
| 650 |
|
4 |
|a Journal Article
|
| 650 |
|
4 |
|a Research Support, Non-U.S. Gov't
|
| 650 |
|
4 |
|a Ulva prolifera
|
| 650 |
|
4 |
|a ammonium
|
| 650 |
|
4 |
|a expression analysis
|
| 650 |
|
4 |
|a gene cloning
|
| 650 |
|
4 |
|a green tide
|
| 650 |
|
4 |
|a nitrate
|
| 650 |
|
4 |
|a nitrate reductase
|
| 650 |
|
7 |
|a Algal Proteins
|2 NLM
|
| 650 |
|
7 |
|a Nitrate Reductase
|2 NLM
|
| 650 |
|
7 |
|a EC 1.7.99.4
|2 NLM
|
| 700 |
1 |
|
|a Wang, Hao Zhe
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Wu, Chun Hui
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Fu, Hui Hui
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Jiang, Peng
|e verfasserin
|4 aut
|
| 773 |
0 |
8 |
|i Enthalten in
|t Journal of phycology
|d 1966
|g 53(2017), 5 vom: 10. Okt., Seite 1035-1043
|w (DE-627)NLM098182994
|x 1529-8817
|7 nnns
|
| 773 |
1 |
8 |
|g volume:53
|g year:2017
|g number:5
|g day:10
|g month:10
|g pages:1035-1043
|
| 856 |
4 |
0 |
|u http://dx.doi.org/10.1111/jpy.12556
|3 Volltext
|
| 912 |
|
|
|a GBV_USEFLAG_A
|
| 912 |
|
|
|a SYSFLAG_A
|
| 912 |
|
|
|a GBV_NLM
|
| 912 |
|
|
|a GBV_ILN_21
|
| 912 |
|
|
|a GBV_ILN_40
|
| 912 |
|
|
|a GBV_ILN_72
|
| 912 |
|
|
|a GBV_ILN_176
|
| 912 |
|
|
|a GBV_ILN_350
|
| 951 |
|
|
|a AR
|
| 952 |
|
|
|d 53
|j 2017
|e 5
|b 10
|c 10
|h 1035-1043
|