Cloning and characterization of nitrate reductase gene in Ulva prolifera (Ulvophyceae, Chlorophyta)
© 2017 Phycological Society of America.
| Veröffentlicht in: | Journal of phycology. - 1966. - 53(2017), 5 vom: 10. Okt., Seite 1035-1043 |
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| Weitere Verfasser: | , , , |
| Format: | Online-Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
2017
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| Zugriff auf das übergeordnete Werk: | Journal of phycology |
| Schlagworte: | Journal Article Research Support, Non-U.S. Gov't Ulva prolifera ammonium expression analysis gene cloning green tide nitrate nitrate reductase Algal Proteins mehr... |
| Zusammenfassung: | © 2017 Phycological Society of America. Ulva spp. dominates green tides around the world, which are occurring at an accelerated rate. The competitive nitrogen assimilation efficiency in Ulva is suggested to result in ecological success against other seaweeds. However, molecular characterization of genes involved in nitrogen assimilation has not been conducted. Here, we describe the identification of the nitrate reductase (NR) gene from a green seaweed Ulva prolifera, an alga which is responsible for the world's largest green tide in the Yellow Sea. Using rapid amplification of cDNA ends and genome walking, the NR gene from U. prolifera (UpNR) was cloned, which consisted of six introns and seven exons encoding 863 amino acids. According to sequence alignment, the NR in U. prolifera was shown to possess all five essential domains and 21 key invariant residues in plant NRs. The GC content of third codon position of UpNR (82.75%) was as high as those of green microalgae, and the intron number supported a potential loss issue from green microalga to land plant. Real-time quantitative PCR results showed that UpNR transcript level was induced by nitrate and repressed by ammonium, which could not be removed by addition of extra nitrate, indicating that U. prolifera preferred ammonium to nitrate. Urea would not repress NR transcription by itself, while it weakened the induction effect of nitrate, implying it possibly inhibited nitrate uptake rather than nitrate reduction. These results suggest the use of UpNR as a gene-sensor to probe the N assimilation process in green tides caused by Ulva |
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| Beschreibung: | Date Completed 14.05.2018 Date Revised 03.12.2018 published: Print-Electronic GENBANK: SRX151604, XP_001696697, KY438973, XP_002955156, AAL79356, ACF22999, NP_177899, XM_001696645, CVU39931, XM_005844731, XM_005647773, X64136, AB232049, NM_106425, XM_015794814 Citation Status MEDLINE |
| ISSN: | 1529-8817 |
| DOI: | 10.1111/jpy.12556 |