Purification of single-stranded DNA by co-polymerization with acrylamide and electrophoresis

Purification of single-stranded DNA by co-polymerization with acrylamide and electrophoresis

Single-stranded DNA (ssDNA) oligonucleotides are useful as aptamers, hybridization probes and for emerging applications in DNA nanotechnology. Current methods to purify ssDNA require both a strand-separation step and a separate size-separation step but may still leave double-stranded DNA (dsDNA) imp...

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Veröffentlicht in:BioTechniques. - 1988. - 62(2017), 6 vom: 01. Juni, Seite 275-282
1. Verfasser: Damase, Tulsi Ram (VerfasserIn)
Weitere Verfasser: Ellington, Andrew D, Allen, Peter B
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2017
Zugriff auf das übergeordnete Werk:BioTechniques
Schlagworte:Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. DNA aptamer purification single-strand generation Aptamers, Nucleotide DNA Primers DNA, Single-Stranded mehr... Immobilized Nucleic Acids Acrylamide 20R035KLCI
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