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231225s2017 xx |||||o 00| ||eng c |
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7 |
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|a 10.2144/000114557
|2 doi
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|a pubmed24n0910.xml
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|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Damase, Tulsi Ram
|e verfasserin
|4 aut
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| 245 |
1 |
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|a Purification of single-stranded DNA by co-polymerization with acrylamide and electrophoresis
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|c 2017
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 15.03.2018
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|a Date Revised 23.10.2019
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|a published: Electronic
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|a Citation Status MEDLINE
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|a Single-stranded DNA (ssDNA) oligonucleotides are useful as aptamers, hybridization probes and for emerging applications in DNA nanotechnology. Current methods to purify ssDNA require both a strand-separation step and a separate size-separation step but may still leave double-stranded DNA (dsDNA) impurities in the sample. Here, we use commercially available acrydite DNA primers to immobilize one strand of a PCR product within a polyacrylamide matrix. Electrophoresis moves the non-crosslinked DNA into the gel where the single-stranded product of desired size can be recovered. Our results show this method produces high yields of pure ssDNA
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|a Journal Article
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|a Research Support, N.I.H., Extramural
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|a Research Support, Non-U.S. Gov't
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| 650 |
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4 |
|a Research Support, U.S. Gov't, Non-P.H.S.
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| 650 |
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4 |
|a DNA
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| 650 |
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4 |
|a aptamer purification
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| 650 |
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4 |
|a single-strand generation
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| 650 |
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7 |
|a Aptamers, Nucleotide
|2 NLM
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| 650 |
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7 |
|a DNA Primers
|2 NLM
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| 650 |
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7 |
|a DNA, Single-Stranded
|2 NLM
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| 650 |
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7 |
|a Immobilized Nucleic Acids
|2 NLM
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| 650 |
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7 |
|a Acrylamide
|2 NLM
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| 650 |
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7 |
|a 20R035KLCI
|2 NLM
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| 700 |
1 |
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|a Ellington, Andrew D
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Allen, Peter B
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t BioTechniques
|d 1988
|g 62(2017), 6 vom: 01. Juni, Seite 275-282
|w (DE-627)NLM012627046
|x 1940-9818
|7 nnns
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| 773 |
1 |
8 |
|g volume:62
|g year:2017
|g number:6
|g day:01
|g month:06
|g pages:275-282
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| 856 |
4 |
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|u http://dx.doi.org/10.2144/000114557
|3 Volltext
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